Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13

被引：91

作者：

Abudayyeh, Omar O. ^{[1
,2
,3
,4
,5
]}

Gootenberg, Jonathan S. ^{[1
,2
,3
,4
,6
]}

Kellner, Max J. ^{[1
]}

Zhang, Feng ^{[1
,2
,3
,4
]}

机构：

[1] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[2] MIT, McGovern Inst Brain Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[3] MIT, Dept Brain & Cognit Sci, E25-618, Cambridge, MA 02139 USA

[4] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, Dept Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

来源：

CRISPR JOURNAL | 2019年 / 2卷 / 03期

关键词：

D O I：

10.1089/crispr.2019.0011

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identification. Here, we use a modified version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (similar to 15 min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This field-ready SHERLOCK platform with color-based lateral flow readout can be applied for detection and quantitation of genes in a range of agricultural applications.

引用

页码：165 / 171

页数：7

共 31 条

[1] RNA targeting with CRISPR-Cas13 [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Essletzbichler, Patrick ;

Han, Shuo ;

Joung, Julia ;

Belanto, Joseph J. ;

Verdine, Vanessa ;

Cox, David B. T. ;

Kellner, Max J. ;

Regev, Aviv ;

Lander, Eric S. ;

Voytas, Daniel F. ;

Ting, Alice Y. ;

Zhang, Feng .

NATURE, 2017, 550 (7675) :280-+

[2] C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Konermann, Silvana ;

Joung, Julia ;

Slaymaker, Ian M. ;

Cox, David B. T. ;

Shmakov, Sergey ;

Makarova, Kira S. ;

Semenova, Ekaterina ;

Minakhin, Leonid ;

Severinov, Konstantin ;

Regev, Aviv ;

Lander, Eric S. ;

Koonin, Eugene V. ;

Zhang, Feng .

SCIENCE, 2016, 353 (6299)

[3] Development of methodologies for virus detection in soybean and wheat seeds [J].

Botelho, Stephanie R. A. ;

Martins, Thais P. ;

Duarte, Macaria F. ;

Barbosa, Andreza V. ;

Lau, Douglas ;

Fernandes, Fernanda R. ;

Sanches, Marcio M. .

METHODSX, 2016, 3 :62-68

[4] Development of a Rapid Point-of-Use DNA Test for the Screening of Genuity® Roundup Ready 2 Yield® Soybean in Seed Samples [J].

Chandu, Dilip ;

Paul, Sudakshina ;

Parker, Mathew ;

Dudin, Yelena ;

King-Sitzes, Jennifer ;

Perez, Tim ;

Mittanck, Don W. ;

Shah, Manali ;

Glenn, Kevin C. ;

Piepenburg, Olaf .

BIOMED RESEARCH INTERNATIONAL, 2016, 2016

[5] Altering the Gut Microbiome of Cattle: Considerations of Host-Microbiome Interactions for Persistent Microbiome Manipulation [J].

Clemmons, Brooke A. ;

Voy, Brynn H. ;

Myer, Phillip R. .

MICROBIAL ECOLOGY, 2019, 77 (02) :523-536

[6] RNA editing with CRISPR-Cas13 [J].

Cox, David B. T. ;

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Franklin, Brian ;

Kellner, Max J. ;

Joung, Julia ;

Zhang, Feng .

SCIENCE, 2017, 358 (6366) :1019-1027

[7] Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection [J].

East-Seletsky, Alexandra ;

O'Connell, Mitchell R. ;

Knight, Spencer C. ;

Burstein, David ;

Cate, Jamie H. D. ;

Tjian, Robert ;

Doudna, Jennifer A. .

NATURE, 2016, 538 (7624) :270-+

[8] Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6 [J].

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Kellner, Max J. ;

Joung, Julia ;

Collins, James J. ;

Zhang, Feng .

SCIENCE, 2018, 360 (6387) :439-+

[9] Nucleic acid detection with CRISPR-Cas13a/C2c2 [J].

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Lee, Jeong Wook ;

Essletzbichler, Patrick ;

Dy, Aaron J. ;

Joung, Julia ;

Verdine, Vanessa ;

Donghia, Nina ;

Daringer, Nichole M. ;

Freije, Catherine A. ;

Myhrvold, Cameron ;

Bhattacharyya, Roby P. ;

Livny, Jonathan ;

Regev, Aviv ;

Koonin, Eugene V. ;

Hung, Deborah T. ;

Sabeti, Pardis C. ;

Collins, James J. ;

Zhang, Feng .

SCIENCE, 2017, 356 (6336) :438-+

[10] Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method [J].

Guan, Xiaoyan ;

Guo, Jinchao ;

Shen, Ping ;

Yang, Litao ;

Zhang, Dabing .

FOOD ANALYTICAL METHODS, 2010, 3 (04) :313-320

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