Real-Time Detection of HIV-2 by Reverse Transcription-Loop-Mediated Isothermal Amplification

被引:12
作者
Curtis, Kelly A. [1 ]
Niedzwiedz, Philip L. [1 ]
Youngpairoj, Ae S. [1 ]
Rudolph, Donna L. [1 ]
Owen, S. Michele [1 ]
机构
[1] Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
关键词
IMMUNODEFICIENCY-VIRUS TYPE-2; INFECTION; UPDATE;
D O I
10.1128/JCM.00935-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device.
引用
收藏
页码:2674 / 2676
页数:3
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