Development of a CAPS marker for the Pvr4 locus:: A tool for pyramiding potyvirus resistance genes in pepper

被引：60

作者：

Caranta, C ^{[1
]}

Thabuis, A ^{[1
]}

Palloix, A ^{[1
]}

机构：

[1] INRA, F-84143 Montfavet, France

来源：

GENOME | 1999年 / 42卷 / 06期

关键词：

amplified fragment length polymorphism markers; bulked segregant analysis; Capsicum annuum; marker-assisted selection; potyvirus resistance;

D O I：

10.1139/gen-42-6-1111

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Pvr4 resistance gene in pepper confers a complete resistance to the three pathotypes of potato virus Y (PVY) and to pepper mottle virus (PepMoV). In order to use this gene in a marker-assisted selection (MAS) program and to permit the pyramiding of several potyvirus resistance genes in the same cultivar, tightly linked amplified fragment length polymorphism (AFLP) markers were obtained by the bulked segregant analysis method. Eight linked AFLP markers were mapped in an interval from 2.1 +/- 0.8 to 13.8 +/- 2.9 cM around this locus. The closest codominant AFLP marker was converted into a codominant CAPS (cleaved amplified polymorphic sequence) marker using data from the alignment of the two allele sequences. We have further characterized the relevance of the CAPS marker for MAS programs in different pepper breeding lines.

引用

页码：1111 / 1116

页数：6

共 25 条

[11]

GEBRESELASSIE K, 1985, AGRONOMIE, V5, P621

[12]

Green S. K., 1991, AVRDC TECH B, V18

[13] A PROCEDURE FOR MAPPING ARABIDOPSIS MUTATIONS USING CODOMINANT ECOTYPE-SPECIFIC PCR-BASED MARKERS [J].