Microtiter method to test the sensitivity of Botrytis cinerea to boscalid

被引：93

作者：

Stammler, G. ^{[1
]}

Speakman, J. ^{[1
]}

机构：

[1] BASF Aktiengesellsch, D-67117 Limburgerhof, Germany

来源：

JOURNAL OF PHYTOPATHOLOGY | 2006年 / 154卷 / 7-8期

关键词：

boscalid; baseline sensitivity; fungicide resistance;

D O I：

10.1111/j.1439-0434.2006.01139.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In 2000, 137 strains of Botrytis cinerea were isolated from different plant hosts worldwide. The isolates had not been exposed to boscalid and were therefore suitable to establish a baseline sensitivity. In order to determine the sensitivity of these isolates, an appropriate microtiter assay was developed. YBA-medium was the medium of choice, as it allowed sufficient growth but did not lead to false positive results. ED50-values ranged from 0.01 to 0.21 ppm, the ED98-values from 0.1 to 2.21 ppm. No growth was observed at concentrations > 3 ppm.

引用

页码：508 / 510

页数：3

共 7 条

[1]

BENYEPHET Y, 1975, PHYTOPATHOLOGY, V65, P563, DOI 10.1094/Phyto-65-563

[2]

BOCHOW H, 1971, Acta Phytopathologica Academiae Scientiarum Hungaricae, V6, P399

[3] 3 GENES DETERMINE CARBOXIN SENSITIVITY OF MITOCHONDRIAL SUCCINATE OXIDATION IN ASPERGILLUS-NIDULANS [J].