The plasma membrane-associated NADH oxidase of spinach leaves responds to blue light

被引:9
作者
Morré, DJ
Penel, C
Greppin, H
Morré, DM
机构
[1] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA
[2] Univ Geneva, Lab Physiol Vegetale, CH-1211 Geneva 4, Switzerland
[3] Purdue Univ, Dept Foods & Nutr, W Lafayette, IN 47907 USA
关键词
physiology; NADH oxidase (NOX); plasma membrane; blue light; biological clock; periodicity; spinach (Spinacia oleracea);
D O I
10.1086/340543
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plasma membrane-associated NADH oxidase (NOX) of spinach leaf disks is characterized by oscillations in activity with a regular period length of ca. 24 min. Within a single population of plants exposed to light at the same time, NOX activities of all plants function synchronously. Exposure of plants transferred from darkness to blue light (495 nm, 2 min, 50 mum mol m(-2) s(-1)) resulted in a complex response pattern but with a new maximum in the rate of NOX activity 36 (24 + 12) min after illumination and then with maxima in the rate of NOX activity every 24 min thereafter. Transient maxima in NOX activity were observed as well after 9.3 +/- 1.4 and 20.7 +/- 2.1 min. The blue light response differed from the response to red (650 nm, 10 min, 50 mum mol m(-2) s(-1)) or white light where activity maxima were initiated 12 min after the light exposure followed by maxima every 24 min thereafter. Green or yellow light was ineffective. The light response was independent of the time in the 24-min NOX cycle when the light was given. The net effects of blue and red light were ultimately the same with a new maximum in the rate of NOX activity at 12 + 24 = 36 min (and every 24 min thereafter), but the mechanisms appear to be distinct.
引用
收藏
页码:543 / 547
页数:5
相关论文
共 20 条
[1]  
Barr R, 2000, ARCH SCI, V53, P225
[2]   The plasma membrane NADH oxidase of soybean has vitamin K1 hydroquinone oxidase activity [J].
Bridge, A ;
Barr, R ;
Morré, DJ .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1463 (02) :448-458
[3]   Photoreceptors in plant photomorphogenesis to date. Five phytochromes, two cryptochromes, one phototropin, and one superchrome [J].
Briggs, WR ;
Olney, MA .
PLANT PHYSIOLOGY, 2001, 125 (01) :85-88
[4]   AUXIN-STIMULATED NADH OXIDASE PURIFIED FROM PLASMA-MEMBRANE OF SOYBEAN [J].
BRIGHTMAN, AO ;
BARR, R ;
CRANE, FL ;
MORRE, DJ .
PLANT PHYSIOLOGY, 1988, 86 (04) :1264-1269
[5]   Blue light sensing in higher plants [J].
Christie, JM ;
Briggs, WR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (15) :11457-11460
[6]   NADH oxidase activity present on both the external and internal surfaces of soybean plasma membranes [J].
DeHahn, T ;
Barr, R ;
Morre, DJ .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1328 (02) :99-108
[7]   Cryptochromes are required for phytochrome signaling to the circadian clock but not for rhythmicity [J].
Devlin, PF ;
Kay, SA .
PLANT CELL, 2000, 12 (12) :2499-2509
[8]   Oxidation of NADH by intact segments of soybean hypocotyls and stimulation by 2,4-D [J].
Hicks, C ;
Morré, DJ .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1375 (1-2) :1-5
[9]   The plasma membrane NADH oxidase of HeLa cells has hydroquinone oxidase activity [J].
Kishi, T ;
Morré, DM ;
Morré, DJ .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1999, 1412 (01) :66-77
[10]   Functional interaction of phytochrome B and cryptochrome 2 [J].
Más, P ;
Devlin, PF ;
Panda, S ;
Kay, SA .
NATURE, 2000, 408 (6809) :207-211