A method for estimation of urease activity in gastric mucosa biopsy specimens and Helicobacter pylori cell suspensions

被引：0

作者：

Vartanova, NO ^{[1
]}

Arzumanyan, VG ^{[1
]}

Basnak'yan, IA ^{[1
]}

Kaverina, KG ^{[1
]}

Chulok, TA ^{[1
]}

机构：

[1] Russian Acad Med Sci, II Metchnikoff Inst Vaccines & Sera, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2002年 / 133卷 / 02期

关键词：

Helicobacter pylori; biopsy specimens; urease activity;

D O I：

10.1023/A:1015523611397

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A method for measuring urease activity in biopsy specimens and Helicobacter pylori cultures from these specimens is proposed. The method is based on measurement (with a portable pH-meter) of the rate of pH changes in a reaction mixture consisting of buffer, substrate (urea), and biopsy specimen or bacterial cells. This method revealed that urease activity of biopsy specimens correlated with that of H. pylori suspension in the same experiment. High urease activity was found in biopsy specimens containing the greatest number of Helicobacter cells; only one of 14 specimens free of H. pylori cells showed no urease activity. Introduction of this method into clinical practice will help to evaluate the contribution of H. pylori to the pathological process.

引用

页码：199 / 201

页数：3