Protease-activated receptor 2 mediates interleukin-8 and intercellular adhesion molecule-1 expression in response to Aggregatibacter actinomycetemcomitans

被引：10

作者：

Shimada, T. ^{[2
]}

Sugano, N. ^{[1
,3
]}

Ikeda, K.

Shimada, K.

Iizuka, T.

Ito, K. ^{[3
]}

机构：

[1] Nihon Univ, Sch Dent, Dept Periodontol, Chiyoda Ku, Tokyo 1018310, Japan

[2] Nihon Univ, Grad Sch Dent, Tokyo 1018310, Japan

[3] Nihon Univ, Sch Dent, Res Ctr, Div Adv Dent Treatment, Tokyo 1018310, Japan

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 2009年 / 24卷 / 04期

关键词：

Aggregatibacter actinomycetemcomitans; intercellular adhesion molecule-1; interleukin-8; protease-activated receptor 2; GINGIVAL EPITHELIAL-CELLS; FUNCTIONAL THROMBIN RECEPTOR; HUMAN PERIODONTAL-DISEASE; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; VIRULENCE FACTORS; SECRETION; PATHOGENESIS; INFLAMMATION; ASTROCYTES;

D O I：

10.1111/j.1399-302X.2009.00507.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Introduction: We investigated the mechanisms by which extracts of Aggregatibacter actinomycetemcomitans affect the inflammatory response in gingival epithelial cells. Methods: Human gingival cells (Ca9-22) were cultured in bacterial extracts prepared from A. actinomycetemcomitans ATCC 29522. The cells were pretreated with protease inhibitors or transfected with small interfering RNA (siRNA) specific for protease-activated receptor 2 (PAR-2). Results: The pretreatment of cells with serine protease inhibitors significantly inhibited A. actinomycetemcomitans extract-induced expression of interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) at both the messenger RNA and protein levels. In addition, A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression was significantly decreased in PAR-2/siRNA-transfected cells. Conclusions: A. actinomycetemcomitans extract-induced IL-8 and ICAM-1 expression in gingival epithelial cells is mediated by PAR-2.

引用

页码：285 / 291

页数：7

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