Membrane proteins of Aedes aegypti larvae bind toxins Cry4B and Cry11A of Bacillus thuringiensis ssp israelensis

被引：0

作者：

Krieger, IV ^{[1
]}

Revina, LP ^{[1
]}

Kostina, LI ^{[1
]}

Buzdin, AA ^{[1
]}

Zalunin, IA ^{[1
]}

Chestukhina, GG ^{[1
]}

Stepanov, VM ^{[1
]}

机构：

[1] Moscow Genet & Select Ind Microorganisms Inst, Moscow 113545, Russia

来源：

BIOCHEMISTRY-MOSCOW | 1999年 / 64卷 / 10期

关键词：

delta-endotoxins; Bacillus thuringiensis; receptors; affinity chromatography;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins of molecular weight 65 and 62 kD and having affinity for toxins Cry4B and Cry11A produced by Bacillus thuringiensis ssp. israelensis have been isolated from brush border membranes of Aedes aegypti larvae using affinity chromatography. Using a ligand blotting technique, we show that the: binding of these proteins to the biotinylated toxins is reversible and that the two toxins compete for binding to the two proteins. These proteins are likely to be Cry4B and Cry11A toxin receptors in gut epithelial cells of Aedes aegypti larvae.

引用

页码：1163 / 1168

页数：6

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