Membrane proteins of Aedes aegypti larvae bind toxins Cry4B and Cry11A of Bacillus thuringiensis ssp israelensis

被引：0

作者：

Krieger, IV ^{[1
]}

Revina, LP ^{[1
]}

Kostina, LI ^{[1
]}

Buzdin, AA ^{[1
]}

Zalunin, IA ^{[1
]}

Chestukhina, GG ^{[1
]}

Stepanov, VM ^{[1
]}

机构：

[1] Moscow Genet & Select Ind Microorganisms Inst, Moscow 113545, Russia

来源：

BIOCHEMISTRY-MOSCOW | 1999年 / 64卷 / 10期

关键词：

delta-endotoxins; Bacillus thuringiensis; receptors; affinity chromatography;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins of molecular weight 65 and 62 kD and having affinity for toxins Cry4B and Cry11A produced by Bacillus thuringiensis ssp. israelensis have been isolated from brush border membranes of Aedes aegypti larvae using affinity chromatography. Using a ligand blotting technique, we show that the: binding of these proteins to the biotinylated toxins is reversible and that the two toxins compete for binding to the two proteins. These proteins are likely to be Cry4B and Cry11A toxin receptors in gut epithelial cells of Aedes aegypti larvae.

引用

页码：1163 / 1168

页数：6

共 50 条

[1] Interaction of 65- and 62-kD Proteins from the Apical Membranes of the Aedes aegypti Larvae Midgut Epithelium with Cry4B and Cry11A Endotoxins of Bacillus thuringiensis
A. A. Buzdin
L. P. Revina
L. I. Kostina
I. A. Zalunin
G. G. Chestukhina
Biochemistry (Moscow), 2002, 67 : 540 - 546
[2] Interaction of 65- and 62-kD proteins from the apical membranes of the Aedes aegypti larvae midgut epithelium with Cry4B and Cry11A endotoxins of Bacillus thuringiensis
Buzdin, AA
Revina, LP
Kostina, LI
Zalunin, IA
Chestukhina, GG
BIOCHEMISTRY-MOSCOW, 2002, 67 (05) : 540 - 546
[3] Expression of the cry11A gene of Bacillus thuringiensis ssp israelensis in Saccharomyces cerevisiae
Quintana-Castro, R
Ramírez-Suero, M
Moreno-Sanz, F
Ramírez-Lepe, M
CANADIAN JOURNAL OF MICROBIOLOGY, 2005, 51 (02) : 165 - 170
[4] Ex vivo cytotoxicity of the Bacillus thuringiensis Cry4B δ-endotoxin to isolated midguts of Aedes aegypti larvae
Barusrux, S
Sramala, I
Katzenmeier, G
Bunyaratvej, A
Panyim, S
Angsuthanasombat, C
JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2003, 36 (03): : 294 - 298
[5] Knockout of Two Cry-Binding Aminopeptidase N Isoforms Does Not Change Susceptibility of Aedes aegypti Larvae to Bacillus thuringiensis subsp. israelensis Cry4Ba and Cry11Aa Toxins
Wang, Junxiang
Yang, Xiaozhen
He, Huan
Chen, Jingru
Liu, Yuanyuan
Huang, Wanting
Ou, Luru
Yang, Zhaohui
Guan, Xiong
Zhang, Lingling
Wu, Songqing
INSECTS, 2021, 12 (03) : 1 - 11
[6] Interaction of Bacillus thuringiensis svar. israelensis Cry toxins with binding sites from Aedes aegypti (Diptera: Culicidae) larvae midgut
Moreira Beltrao, Henrique de Barros
Neves Lobo Silva-Filha, Maria Helena
FEMS MICROBIOLOGY LETTERS, 2007, 266 (02) : 163 - 169
[7] Identification and characterization of Aedes aegypti aminopeptidase N as a putative receptor of Bacillus thuringiensis Cry11A toxin
Chen, Jianwu
Aimanova, Karlygash G.
Pan, Songqin
Gill, Sarjeet S.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2009, 39 (10) : 688 - 696
[8] Production of Cry11A and Cry11Ba toxins in Bacillus sphaericus confers toxicity towards Aedes aegypti and resistant Culex populations
Servant, P
Rosso, ML
Hamon, S
Poncet, S
Delécluse, A
Rapoport, G
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999, 65 (07) : 3021 - 3026
[9] Cadherin binding is not a limiting step for Bacillus thuringiensis subsp israelensis Cry4Ba toxicity to Aedes aegypti larvae
Rodriguez-Almazan, Claudia
Reyes, Esmeralda Z.
Zuniga-Navarrete, Fernando
Munoz-Garay, Carlos
Gomez, Isabel
Evans, Amy M.
Likitvivatanavong, Supaporn
Bravo, Alejandra
Gill, Sarjeet S.
Soberon, Mario
BIOCHEMICAL JOURNAL, 2012, 443 : 711 - 717
[10] Investigation of the Cry4B–Prohibitin Interaction in Aedes aegypti Cells
Atichat Kuadkitkan
Duncan R. Smith
Colin Berry
Current Microbiology, 2012, 65 : 446 - 454

← 1 2 3 4 5 →