A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis

被引:88
作者
Yuan, Hui [1 ,2 ]
Owsiany, Katherine [2 ]
Sheeja, T. E. [2 ]
Zhou, Xiangjun [1 ,2 ]
Rodriguez, Caroline [2 ]
Li, Yongxi [5 ]
Welsch, Ralf [6 ]
Chayut, Noam [7 ]
Yang, Yong [1 ]
Thannhauser, Theodore W. [1 ]
Parthasarathy, Mandayam V. [3 ]
Xu, Qiang [5 ]
Deng, Xiuxin [5 ]
Fei, Zhangjun [4 ]
Schaffer, Ari [7 ]
Katzir, Nurit [7 ]
Burger, Joseph [7 ]
Tadmor, Yaakov [7 ]
Li, Li [1 ,2 ]
机构
[1] Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA
[2] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA
[3] Cornell Univ, Sch Integrat Plant Sci, Plant Biol Sect, Ithaca, NY 14853 USA
[4] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[5] Huazhong Agr Univ, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China
[6] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany
[7] Agr Res Org, Newe Yaar Res Ctr, IL-30095 Ramat Yishay, Israel
关键词
BETA-CAROTENE; PHYTOENE SYNTHASE; ENHANCED ACCUMULATION; GENETIC-VARIATION; BIOSYNTHESIS; CAULIFLOWER; BIOGENESIS; METABOLISM; SYSTEM; DRIVEN;
D O I
10.1104/pp.15.00971
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Carotenoids are crucial for plant growth and human health. The finding of ORANGE (OR) protein as a pivotal regulator of carotenogenesis offers a unique opportunity to comprehensively understand the regulatory mechanisms of carotenoid accumulation and develop crops with enhanced nutritional quality. Here, we demonstrated that alteration of a single amino acid in a wild-type OR greatly enhanced its ability to promote carotenoid accumulation. Whereas overexpression of OR from Arabidopsis (Arabidopsis thaliana; AtOR) or from the agronomically important crop sorghum (Sorghum bicolor; SbOR) increased carotenoid levels up to 2-fold, expression of AtORHis (R90H) or SbORHis (R104H) variants dramatically enhanced carotenoid accumulation by up to 7-fold in the Arabidopsis calli. Moreover, we found that AtORAla (R90A) functioned similarly to AtORHis to promote carotenoid overproduction. Neither AtOR nor AtORHis greatly affected carotenogenic gene expression. AtORHis exhibited similar interactions with phytoene synthase (PSY) as AtOR in posttranscriptionally regulating PSY protein abundance. AtORHis triggered biogenesis of membranous chromoplasts in the Arabidopsis calli, which shared structures similar to chromoplasts found in the curd of the orange cauliflower (Brassica oleracea) mutant. By contrast, AtOR did not cause plastid-type changes in comparison with the controls, but produced plastids containing larger and electron-dense plastoglobuli. The unique ability of AtORHis in mediating chromoplast biogenesis is responsible for its induced carotenoid overproduction. Our study demonstrates ORHis/Ala as powerful tools for carotenoid enrichment in plants, and provides insights into the mechanisms underlying ORHis-regulated carotenoid accumulation.
引用
收藏
页码:421 / +
页数:24
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