Transcriptome analysis of Sclerotinia ginseng and comparative analysis with the genome of Sclerotinia sclerotiorum

被引:8
|
作者
Wang, Dan [1 ]
Fu, Junfan [1 ]
Zhou, Rujun [1 ]
Li, Zibo [1 ]
Han, Yueling [1 ]
Liu, Xinran [1 ]
机构
[1] Shenyang Agr Univ, Dept Plant Protect, Room 120,Dongling Rd, Shenyang 110866, Liaoning, Peoples R China
关键词
Sclerotiniaceae; Transcriptome profile; De novo assembly; Genome; Phylogenetic analysis; PH; OXALATE; IDENTIFICATION; GENE; POLYGALACTURONASE; ACTIVATION; OMICS; TOOL;
D O I
10.1016/j.pmpp.2018.11.003
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Sclerotinia ginseng is a non-model necrotrophic pathogen responsible for a serious epidemic in the ginseng production area in northeast China. In this study, de novo RNA sequencing was carried out using an Illumina HiSeq (TM) 4000 platform in conjunction with the short-read assembly program Trinity. The S. ginseng transcriptome was compared with that of Sclerotinia sclerotiorum, and species-specific unigenes were annotated with KEGG and GO terms. Phylogenetic analysis of amino acid sequences for four polygalacturonase (pg) genes and a pH-response transcription factor (pac1) homologous gene identified in S. ginseng revealed a close evolutionary relationship with other species of Sclerotiniaceae.
引用
收藏
页码:30 / 41
页数:12
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