Top-down mass spectrometry of histone modifications in sorghum reveals potential epigenetic markers for drought acclimation

被引：14

作者：

Zhou, Mowei ^{[1
]}

Malhan, Neha ^{[1
]}

Ahkami, Amir H. ^{[1
]}

Engbrecht, Kristin ^{[1
]}

Myers, Gabriel ^{[1
]}

Dahlberg, Jeffery ^{[2
]}

Hollingsworth, Joy ^{[2
]}

Sievert, Julie A. ^{[2
]}

Hutmacher, Robert ^{[3
]}

Madera, Mary ^{[4
]}

Lemaux, Peggy G. ^{[4
]}

Hixson, Kim K. ^{[1
]}

Jansson, Christer ^{[1
]}

Pasa-Tolic, Ljiljana ^{[1
]}

机构：

[1] Pacific Northwest Natl Lab, Environm Mol Sci Lab, Richland, WA 99354 USA

[2] UC ANR Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA

[3] Univ Calif Davis, UC Dept Plant Sci, UC ANR Westside Res & Extens Ctr, Five Points, CA 93624 USA

[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

来源：

METHODS | 2020年 / 184卷

关键词：

Drought; Epigenetics; Histone clipping; Post-translational modifications; Sorghum; Top-down mass spectrometry; N-TERMINAL METHYLATION; POSTTRANSLATIONAL MODIFICATIONS; SOFTWARE TOOL; MIDDLE-DOWN; PROTEOMICS; IDENTIFICATION; PROTEINS; VARIANTS; H3; QUANTITATION;

D O I：

10.1016/j.ymeth.2019.10.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed leaves of 48 plants and identified 26 unique histone proteins and 677 unique histone proteoforms (124 full-length and 553 truncated proteoforms). We detected trimethylation on nearly all H2B N-termini where acetylation is commonly expected. In addition, an unexpected modification on H2A histones was assigned to N-pyruvic acid 2-iminylation based on its unique neutral loss of CO2. Interestingly, some of the truncated histones, in particular H4 and H3.2, showed significant changes that correlated with the growth and water conditions. The histone proteoforms could serve as targets in search of chromatin modifiers and ultimately have important ramifications in future attempts of studying plant epigenetic reprogramming under stress.

引用

页码：29 / 39

页数：11

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