Conformational studies of the capsular polysaccharide produced by Neisseria meningitidis group A

被引：14

作者：

Foschiatti, Michela ^{[1
]}

Hearshaw, Meredith ^{[2
]}

Cescutti, Paola ^{[1
]}

Ravenscroft, Neil ^{[2
]}

Rizzo, R. ^{[1
]}

机构：

[1] Univ Trieste, Dept Life Sci, I-34127 Trieste, Italy

[2] Univ Cape Town, Dept Chem, ZA-7701 Rondebosch, South Africa

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Neisseria meningitidis; Capsular polysaccharide; Structure; Conformation; Atomic force microscopy; Vaccines; ATOMIC-FORCE MICROSCOPY;

D O I：

10.1016/j.carres.2009.02.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of different cations on the conformational and morphological properties of the capsular polysaccharide produced by Neisseria meningitidis group A was investigated. Circular dichroism studies showed that the presence of Na+, NH4+ or Ca2+ ions induced different local conformations of the polysaccharide chain through interactions with the phosphodiester group bridging the saccharide residues in the polymer chain. Atomic force microscopy experiments confirmed that the morphology of the polysaccharide chains was different depending on the nature of the counterion. Ammonium ions were associated with the presence of single polymer chains in an elongated conformation. whereas sodium ions favored the folding of the chains into a globular conformation. The addition of calcium ions produced the aggregation of a limited number of globular polysaccharide chains to form a 'toroidal-like' structure. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：940 / 943

页数：4

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