Nociceptive vocalization response in guinea pigs modulated by opioidergic, GABAergic and serotonergic neurotransmission in the dorsal raphe nucleus

被引:8
作者
Ferreira, Mateus Dalbem [1 ]
Menescal-de-Oliveira, Leda [1 ]
机构
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Physiol, BR-14049900 Ribeirao Preto, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Dorsal raphe nucleus; Serotonin; GABAergic system; Opioidergic system; Nociceptive vocalization response; VENTROLATERAL ORBITAL CORTEX; TONIC IMMOBILITY; 5-HYDROXYTRYPTAMINE RELEASE; INDUCED ANTINOCICEPTION; NEURONS; RAT; MORPHINE; PAIN; STIMULATION; ANALGESIA;
D O I
10.1016/j.brainresbull.2014.05.001
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The dorsal raphe nucleus (DRN) is involved in the control of several physiological functions, including nociceptive modulation. This nucleus is one of the main sources of serotonin to the CNS and neuromodulators such as opioids and GABA may be are important for its release. This study evaluated the influence of serotonergic, GABAergic and opioidergic stimulation, as well as their interactions in the DRN, on vocalization nociceptive response during a peripheral noxious stimulus application in guinea pigs. Morphine (1.1 nmol), bicuculline (0.50 nmol) and alpha-methyl-5-HT (1.6 nmol) microinjection on the DRN produces antinociception. The antinociception produced by morphine (1.1 nmol) and alpha-methyl-5-HT (1.6 nmol) into the DRN was blocked by prior microinjection of naloxone (0.7 nmol). The alpha-methyl-5-HT effect blocked by naloxone may indicate the existence of 5-HT2A receptors on enkephalinergic interneurons within the dorsal raphe. Pretreatment with muscimol (0.26 nmol) also prevented the antinociceptive effect caused by morphine (1.1 nmol) when administered alone at the same site, indicating an interaction between GABAergic and opioidergic interneurons. The antinociception produced by bicuculline (0.5 nmol) in the DRN was blocked by prior administration of 8-0H-DPAT (0.5 nmol), a 5-HT1A agonist. This may indicate that the 5-HT autoreceptor activation by 8-OH-DPAT at DRN effector neurons can oppose the bicuculline disinhibition effect applied to the same effectors. Thus, we suggest that 5-HT2 receptor activation in the DRN promotes endorphin/enkephalin release that may disinhibit efferent serotonergic neurons of this present structure by inhibiting GABAergic interneurons, resulting in antinociception. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:21 / 29
页数:9
相关论文
共 56 条
  • [11] GABAERGIC MODULATION OF NOCICEPTIVE THRESHOLD - EFFECTS OF THIP AND BICUCULLINE MICROINJECTED IN THE VENTRAL MEDULLA OF THE RAT
    DROWER, EJ
    HAMMOND, DL
    [J]. BRAIN RESEARCH, 1988, 450 (1-2) : 316 - 324
  • [12] Duggan A.W., 1983, PHARMACOL REV, V352, P19
  • [13] A REINVESTIGATION OF THE ANALGESIC EFFECTS INDUCED BY STIMULATION OF THE PERIAQUEDUCTAL GRAY-MATTER IN THE RAT .1. THE PRODUCTION OF BEHAVIORAL SIDE-EFFECTS TOGETHER WITH ANALGESIA
    FARDIN, V
    OLIVERAS, JL
    BESSON, JM
    [J]. BRAIN RESEARCH, 1984, 306 (1-2) : 105 - 123
  • [14] Role of cholinergic, opioidergic and GABAergic neurotransmission of the dorsal hippocampus in the modulation of nociception in guinea pigs
    Favaroni Mendes, Lys Angela
    Menescal-de-Oliveira, Leda
    [J]. LIFE SCIENCES, 2008, 83 (19-20) : 644 - 650
  • [15] Opioidergic, GABAergic and serotonergic neurotransmission in the dorsal raphe nucleus modulates tonic immobility in guinea pigs
    Ferreira, Mateus Dalbem
    Menescal-de-Oliveira, Leda
    [J]. PHYSIOLOGY & BEHAVIOR, 2012, 106 (02) : 109 - 116
  • [16] Role of dorsal raphe nucleus 5-HT1A and 5-HT2 receptors in tonic immobility modulation in guinea pigs
    Ferreira, Mateus Dalbem
    Menescal-de-Oliveira, Leda
    [J]. BRAIN RESEARCH, 2009, 1285 : 69 - 76
  • [17] THE ROLE OF PERIPHERAL 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E AND 5-HT1F SEROTONERGIC RECEPTORS IN THE REDUCTION OF NOCICEPTION IN RATS
    Granados-Soto, V.
    Arguelles, C. F.
    Rocha-Gonzalez, H. I.
    Godinez-Chaparro, B.
    Flores-Murrieta, F. J.
    Villalon, C. M.
    [J]. NEUROSCIENCE, 2010, 165 (02) : 561 - 568
  • [18] MORPHINE INDUCES AN INCREASE IN EXTRACELLULAR SEROTONIN IN THE RAT DIENCEPHALON
    GRAUER, SM
    TAO, R
    AUERBACH, SB
    [J]. BRAIN RESEARCH, 1992, 599 (02) : 277 - 282
  • [19] GAMMA-AMINOBUTYRIC-ACID AND 5-HYDROXYTRYPTAMINE INTERRELATIONSHIP IN THE RAT NUCLEUS RAPHE DORSALIS - COMBINATION OF AUTORADIOGRAPHIC AND IMMUNOCYTOCHEMICAL TECHNIQUES AT LIGHT AND ELECTRON-MICROSCOPY LEVELS
    HARANDI, M
    AGUERA, M
    GAMRANI, H
    DIDIER, M
    MAITRE, M
    CALAS, A
    BELIN, MF
    [J]. NEUROSCIENCE, 1987, 21 (01) : 237 - 251
  • [20] GABAergic modulation is involved in the ventrolateral orbital cortex 5-HT1A receptor activation-induced antinociception in the rat
    Huo, Fu-Quan
    Qu, Chao-Ling
    Li, Yun-Qing
    Tang, Jing-Shi
    Jia, Hong
    [J]. PAIN, 2008, 139 (02) : 398 - 405