Isolation of alpha-tocopherol (vitamin E) from garlic

被引:12
|
作者
Malik, MN [1 ]
Fenko, MD [1 ]
Shiekh, AM [1 ]
Wisniewski, HM [1 ]
机构
[1] NEW YORK STATE INST BASIC RES DEV DISABIL,DEPT PATHOL NEUROBIOL,STATEN ISL,NY 10314
关键词
vitamin E; garlic; alpha-tocopherol; LDL oxidation;
D O I
10.1021/jf960524b
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Vitamin E (alpha-tocopherol) was isolated from garlic. The method involved homogenization of garlic in phosphate-buffered saline and extraction with heptane in the presence of lithium dodecyl sulfate. An aliquot of the extract, when analyzed by HPLC on a C18 column, yielded a peak with the same retention time as the alpha-tocopherol standard. The presence of alpha-tocopherol was further confirmed by GC/MS. A total of 9.4 mu g of alpha-tocopherol was isolated from 1 g of garlic. Oxidation of low-density lipoprotein was retarded by garlic alpha-tocopherol.
引用
收藏
页码:817 / 819
页数:3
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