The Arabidopsis Ethylene-Insensitive 2 gene is required for lead resistance

被引:79
作者
Cao, Shuqing [1 ]
Chen, Zhengyi [1 ]
Liu, Guoqing [1 ]
Jiang, Li [1 ]
Yuan, Huaibo [1 ]
Ren, Guang [1 ]
Bian, Xiaohui [1 ]
Jian, Hongyong [1 ]
Ma, Xinliang [1 ]
机构
[1] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Anhui, Peoples R China
基金
中国国家自然科学基金;
关键词
Arabidopsis; Ethylene-Insensitive; 2; gene; Lead; STRESS-RESPONSE; CADMIUM; ETHYLENE; ARABIDOPSIS; PLANTS; ZNTA;
D O I
10.1016/j.plaphy.2008.12.013
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The Arabidopsis Ethylene-Insensitive 2 (EIN2) gene has been shown to be involved in the regulation of abiotic and biotic stresses, including ozone stress, high salt, oxidative stress and disease resistance. However, little is known about the role of EIN2 gene in lead (Pb) resistance in Arabidopsis. In this study, we showed that EIN2 gene is required for Pb(II) resistance in Arabidopsis. EIN2 gene was induced by Pb(II) treatment, and the ein2-1 mutant showed enhanced sensitivity to Pb(II). A higher Pb content was detected in ein2-1 plants than in wild-type plants when subjected to Pb(II) treatment, which was associated, at least in part, with reduction in expression of AtPDR12 gene, a pump excluding Pb(II) and/or Pb(II)-containing toxic compounds from the cytoplasm. Moreover, the ein2-1 mutation also impaired glutathione (GSH)-dependent Pb(II) resistance, which was related to constitutive reduction of express of GSH1 gene involved in GSH synthesis and consequently reduced GSH content. Taken together, all these results suggest that EIN2 gene mediates Pb(II) resistance, at least in part, through two distinct mechanisms, a GSH-dependent mechanism and a GSH-independent AtPDR12-mediated mechanism. (C) 2009 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:308 / 312
页数:5
相关论文
共 21 条
  • [1] EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis
    Alonso, JM
    Hirayama, T
    Roman, G
    Nourizadeh, S
    Ecker, JR
    [J]. SCIENCE, 1999, 284 (5423) : 2148 - 2152
  • [2] Evidence for a role of Ethylene-Insensitive 2 gene in the regulation of the oxidative stress response in Arabidopsis
    Cao, Shuqing
    Jiang, Shaotong
    Zhang, Rongxian
    [J]. ACTA PHYSIOLOGIAE PLANTARUM, 2006, 28 (05) : 417 - 425
  • [3] PHYTOCHELATINS, THE HEAVY-METAL-BINDING PEPTIDES OF PLANTS, ARE SYNTHESIZED FROM GLUTATHIONE BY A SPECIFIC GAMMA-GLUTAMYLCYSTEINE DIPEPTIDYL TRANSPEPTIDASE (PHYTOCHELATIN SYNTHASE)
    GRILL, E
    LOFFLER, S
    WINNACKER, EL
    ZENK, MH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) : 6838 - 6842
  • [4] Cellular mechanisms for heavy metal detoxification and tolerance
    Hall, JL
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2002, 53 (366) : 1 - 11
  • [5] The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance
    Kim, Do-Young
    Bovet, Lucien
    Maeshima, Masayoshi
    Martinoia, Enrico
    Lee, Youngsook
    [J]. PLANT JOURNAL, 2007, 50 (02) : 207 - 218
  • [6] AtATM3 is involved in heavy metal resistance in Arabidopsis
    Kim, DY
    Bovet, L
    Kushnir, S
    Noh, EW
    Martinoia, E
    Lee, Y
    [J]. PLANT PHYSIOLOGY, 2006, 140 (03) : 922 - 932
  • [7] Environmental health - The paradox of lead poisoning prevention
    Lanphear, BP
    [J]. SCIENCE, 1998, 281 (5383) : 1617 - 1618
  • [8] AtPDR12 contributes to lead resistance in arabidopsis
    Lee, M
    Lee, K
    Lee, J
    Noh, EW
    Lee, Y
    [J]. PLANT PHYSIOLOGY, 2005, 138 (02) : 827 - 836
  • [9] Overexpression of Arabidopsis phytochelatin synthase paradoxically leads to hypersensitivity to cadmium stress
    Lee, S
    Moon, JS
    Ko, TS
    Petros, D
    Goldsbrough, PB
    Korban, SS
    [J]. PLANT PHYSIOLOGY, 2003, 131 (02) : 656 - 663
  • [10] A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium
    Li, ZS
    Lu, YP
    Zhen, RG
    Szczypka, M
    Thiele, DJ
    Rea, PA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (01) : 42 - 47