A SURVEY OF DEFORMED WING VIRUS AND ACUTE BEE PARALYSIS VIRUS IN HONEY BEE COLONIES FROM SERBIA USING REAL-TIME RT-PCR

被引：13
作者：
Simeunovic, Predrag [1 ]
Stevanovic, Jevrosima [1 ]
Vidanovic, Dejan [2 ]
Nisavic, Jakov [3 ]
Radovic, Dejan [4 ]
Stanisic, Ljubodrag [1 ]
Stanimirovic, Zoran [1 ]
机构：
[1] Univ Belgrade, Dept Biol, Fac Vet Med, Belgrade, Serbia
[2] Vet Specialist Inst Kraljevo, Kraljevo, Serbia
[3] Univ Belgrade, Dept Microbiol, Fac Vet Med, Belgrade, Serbia
[4] Univ Belgrade, Fac Biol, Belgrade, Serbia
来源：
ACTA VETERINARIA-BEOGRAD | 2014年 / 64卷 / 01期
关键词：
DWV; ABPV; Varroa destructor; Serbian apiaries; QUEEN CELL VIRUS; APIS-MELLIFERA; VERTICAL-TRANSMISSION; VARROA-DESTRUCTOR; SCIENTIFIC NOTE; SACBROOD VIRUS; VIRAL LOAD; PREVALENCE; APIARIES; STRAINS;
D O I：
10.2478/acve-2014-0009
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.
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收藏
页码：81 / 92
页数：12
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