Surface Modification of a Stable CdSeZnS/ZnS Alloy Quantum Dot for Immunoassay

被引：6

作者：

An, Jaehyun ^{[1
]}

Huynh, Kim-Hung ^{[1
]}

Ha, Yuna ^{[1
]}

Jung, Heung Su ^{[2
]}

Kim, Hyung-Mo ^{[1
]}

Kim, Dong-Min ^{[1
]}

Kim, Jaehi ^{[1
]}

Pham, Xuan-Hung ^{[1
]}

Kim, Dong-Eun ^{[1
]}

Ho, Jin-Nyoung ^{[3
]}

Lee, Sangchul ^{[3
]}

Lee, Ho-Young ^{[4
]}

Jeong, Dae Hong ^{[5
]}

Jun, Bong-Hyun ^{[1
]}

机构：

[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea

[2] Co GLOBAL ZEUS, Osan 18364, Gyeonggi Do, South Korea

[3] Seoul Natl Univ, Dept Urol, Bundang Hosp, Seoul 13620, South Korea

[4] Seoul Natl Univ, Dept Nucl Med, Bundang Hosp, Seoul 13620, South Korea

[5] Seoul Natl Univ, Dept Chem Educ, Seoul 151019, South Korea

来源：

JOURNAL OF NANOMATERIALS | 2020年 / 2020卷

基金：

新加坡国家研究基金会;

关键词：

C-REACTIVE PROTEIN; SHELL THICKNESS; FABRICATION; CDSE; BIOCONJUGATION; NANOPARTICLES; PROBES; YIELD;

D O I：

10.1155/2020/4937049

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Quantum dots (QDs) are powerful materials in various bioapplications based on their excellent optical and electronic properties. For the application of various fields of QDs, surface modification of QDs is necessary. However, surface modification in QDs may result in a reduction in quantum yield (QY). This reduction of QY causes many weaknesses in the biological application of QDs. In this study, CdSeZnS/ZnS alloy QDs were used to prepare antibody-conjugated QDs for a sandwich immunoassay. The alloy QDs displayed a QY of 84.5% that was maintained at 83.0% (98.2% of QY was maintained) after surface modification with the anti-rabbit IgG as a model study. Surface-modified QDs successfully detected their corresponding target through antibody-antigen binding. The limit of detection was 1.1x10(2) ng mL(-1) for rabbit IgG.

引用

页数：9

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