Toll-like receptors and high mobility group box 1 in granulosa cells during bovine follicle maturation

被引：10

作者：

Xie, Yingying ^{[1
]}

Zhang, Kang ^{[1
]}

Zhang, Kai ^{[1
]}

Zhang, Jingyan ^{[1
]}

Wang, Lei ^{[1
]}

Wang, Xurong ^{[1
]}

Hu, Xuequan ^{[2
]}

Liang, Zijing ^{[1
]}

Li, Jianxi ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Engn & Technol Res Ctr Tradit Chinese Vet Med Gan, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou, Gansu, Peoples R China

[2] Gansu Agr Univ, Coll Vet Med, Lanzhou, Gansu, Peoples R China

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2020年 / 235卷 / 04期

基金：

中国国家自然科学基金;

关键词：

bovine; FSH; HMGB1; ovarian granulosa cells; TLR2; TLR4; NF-KAPPA-B; PATTERN-RECOGNITION RECEPTORS; STIMULATING-HORMONE RECEPTOR; MESSENGER-RNA EXPRESSION; DOUBLE-STRANDED-RNA; INNATE IMMUNITY; HMGB1; MECHANISMS; INFLAMMATION; ACTIVATION;

D O I：

10.1002/jcp.29234

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Toll-like receptors (TLRs) are present in the ovaries and reproductive tract of various mammals. The biological function of TLR during ovulation is one of the main contents in the research of reproductive immunology. In this study, we found that messenger RNA levels of TLR1-TLR10 in granulosa cells were different, and TLRs and high mobility group box 1 (HMGB1) in granulosa cells of large follicles were significantly higher than those of small and middle follicles. Coimmunoprecipitation results showed that HMGB1 interacts with TLR2 in granulosa cells, especially large follicles. The result of immunohistochemistry showed that TLRs and HMGB1 were present in granulosa cell layer of ovarian follicles. We also found 25 mIU/ml follicle-stimulating hormone (FSH) significantly upregulated the expression of TLRs and HMGB1. These results suggest that TLR2/4 and HMGB1 in granulosa cells may be involved in the ovarian innate immune and ovarian follicular maturation, regulated by FSH. However, further research of the function and mechanisms of TLRs and HMGB1 in granulosa cells are needed.

引用

页码：3447 / 3462

页数：16

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