Shifting the paradigm: new approaches for characterizing and classifying neurons

被引：20

作者：

Bernard, Amy ^{[1
]}

Sorensen, Staci A. ^{[1
]}

Lein, Ed S. ^{[1
]}

机构：

[1] Allen Inst Brain Sci, Seattle, WA 98103 USA

来源：

CURRENT OPINION IN NEUROBIOLOGY | 2009年 / 19卷 / 05期

关键词：

TRANSLATIONAL PROFILING APPROACH; GENETICALLY TARGETED NEURONS; GENE-EXPRESSION ATLAS; CNS CELL-TYPES; IN-VIVO; TRANSGENIC MICE; TRANSCRIPTOME ANALYSIS; MAMMALIAN NEURONS; NETWORK ACTIVITY; NEURAL CIRCUITS;

D O I：

10.1016/j.conb.2009.09.010

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Efforts to characterize and classify the cellular components of the nervous system have a rich history in modern neuroscience, and closely mirror the development of new techniques to assay cellular properties. Recent advances in high-throughput histology, genetics and neuroinformatics hold great promise for systematic and reproducible measurement and community databasing of cellular properties. In particular, transgenic approaches to reproducibly target and manipulate specific cell types in mice are rapidly advancing. Cre recombinase-based approaches in particular allow the coupling of cell type specificity with a wide variety of genetic tools for visualization, molecular profiling, tract tracing and functional manipulation. The reproducible multimodal characterization allowed by these transgenics provides a means to classify, building cellular taxonomies based on measurement of many phenotypic properties, as well as manipulate, moving beyond classification to understand the functional role of specific cell types and circuits in complex behaviors.

引用

页码：530 / 536

页数：7

共 54 条

[41] GABAergic neurons are less selective to stimulus orientation than excitatory neurons in layer II/III of visual cortex, as revealed by in vivo functional Ca2+ imaging in transgenic mice [J].