A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

被引：285

作者：

Liu, Zhixue ^{[1
,2
]}

Zhou, Xin ^{[1
,2
]}

Miao, Yu ^{[1
,2
]}

Hu, Ying ^{[3
]}

Kwon, Nahyun ^{[3
]}

Wu, Xue ^{[1
,2
]}

Yoon, Juyoung ^{[3
]}

机构：

[1] Yanbian Univ, Key Lab Nat Resources Changbai Mt & Funct Mol, Minist Educ, Yanji 133002, Peoples R China

[2] Yanbian Univ, Res Ctr Chem Biol, Dept Chem, Yanji 133002, Peoples R China

[3] Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2017年 / 56卷 / 21期

基金：

新加坡国家研究基金会;

关键词：

antioxidants; fluorescent probes; gluthionine; ratiometric probes; redox homeostasis; ASSISTED ELECTROSTATIC ATTRACTION; SELECTIVE DETECTION; IN-VIVO; EMISSION CHANNELS; MICHAEL ADDITION; CYSTEINE; CELLS; GSH; THIOLS; SENSOR;

D O I：

10.1002/anie.201702114

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (K-d) of 2.59 mM and a fast response time (t(1/2) = 5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40 +/- 0.87 mM).

引用

页码：5812 / 5816

页数：5

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