Detection of chemiluminescence using an amorphous silicon photodiode

被引:25
作者
Pimentel, Alexandra C. [1 ]
Pereira, A. T.
Chu, V.
Prazeres, D. M. F.
Conde, J. P.
机构
[1] INESC, Microsistemas & Nanotecnol, Lisbon, Portugal
[2] Univ Tecn Lisboa, Ctr Engn Biol & Quim, Inst Super Tecn, Lisbon, Portugal
[3] Univ Tecn Lisboa, Dept Chem & Biol Engn, Inst Super Tecn, Lisbon, Portugal
关键词
chemiluminescence; horseradish peroxidase (HRP); photodiode; protein detection;
D O I
10.1109/JSEN.2006.890144
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
A hydrogenated amorphous silicon photodiode is used for the integrated, real-time detection of chemiluminescent reactions in solution. The light emission at 425 nm, resulting from the horseradish peroxidase-catalyzed oxidation of luminol, is detected by a p-i-n amorphous silicon photodiode operated at zero bias. The sensitivity achieved is in the range of nanomole of horseradish peroxidase per liter.
引用
收藏
页码:415 / 416
页数:2
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