Photoacclimation and productivity of Chlamydomonas reinhardtii grown in fluctuating light regimes which simulate outdoor algal culture conditions

被引:37
作者
Yarnold, Jennifer [1 ]
Ross, Ian L. [1 ]
Hankamer, Ben [1 ]
机构
[1] Univ Queensland, Inst Mol Biosci, 306 Carmody Rd, Brisbane, Qld 4072, Australia
来源
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2016年 / 13卷
关键词
Microalgae; Chlamydomonas reinhardtii; Photoacclimation; Non-photochemical quenching; Fluctuating light; Mass culture; LHCSR3; Photosynthesis; Predictive model; CYCLIC ELECTRON FLOW; HARVESTING PROTEIN; GENE-EXPRESSION; SOLAR-ENERGY; GREEN-ALGA; PHOTOSYNTHESIS; MODEL; MICROALGAE; BIOMASS; PHOTOINHIBITION;
D O I
10.1016/j.algal.2015.11.001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Outdoor microalgae systems are a promising platform for fuels and chemicals, but are currently limited by relatively low productivities. This study investigated the effects of photoacclimation on the productivity of Chlamydomonas reinhardtii grown under fluctuating light regimes which simulate well-mixed cultures in outdoor reactors. Simulations represented cells cycling between high light and dark zones in high-density (HDFluc, light fraction (LF)= 0.5) and low-density (LDFluc, LF= 0.9) cultures. Each fluctuating treatment was controlled by cultures grown under non-fluctuating light of the same hourly average irradiance (HDAvg and LDAvg) to differentiate between light dosage and regime. The large dark fraction of HDFluc resulted in a low-light acclimated phenotype displaying up-regulation of light harvesting pigments and low NPQ caused by reduced levels of the dissipative protein LHCSR3. All other treatments led to high-light acclimation phenotypes. HDFluc showed an estimated three-fold lower biomass yield relative to light absorbed and significant reductions in the quantum yield of PSII compared to HDAvg. This suggests that during high lightp eriods of fluctuating cycles, higher absorption and an inability to safely dissipate excess light, resulted in greater photodamage and respiration required for repair. A framework for including these findings in predictive modelling of mass cultures is presented. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:182 / 194
页数:13
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