Overexpression of the NDR1/HIN1-Like Gene NHL6 Modifies Seed Germination in Response to Abscisic Acid and Abiotic Stresses in Arabidopsis

被引：43

作者：

Bao, Yan ^{[1
,2
,3
,4
]}

Song, Wei-Meng ^{[2
]}

Pan, Jing ^{[2
]}

Jiang, Chun-Mei ^{[2
]}

Srivastava, Renu ^{[3
,4
]}

Li, Bei ^{[2
]}

Zhu, Lu-Ying ^{[1
]}

Su, Hong-Yan ^{[1
]}

Gao, Xiao-Shu ^{[2
]}

Liu, Hua ^{[2
]}

Yu, Xiang ^{[2
]}

Yang, Lei ^{[5
]}

Cheng, Xian-Hao ^{[1
]}

Zhang, Hong-Xia ^{[1
,2
]}

机构：

[1] Ludong Univ, Coll Agr, 186 Hongqizhong Rd, Yantai 264025, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, 300 Fenglin Rd, Shanghai 200032, Peoples R China

[3] Iowa State Univ, Inst Plant Sci, Ames, IA 50011 USA

[4] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA

[5] Nanjing Univ, Coll Life Sci, 22 Hankou Rd, Nanjing 210093, Jiangsu, Peoples R China

来源：

PLOS ONE | 2016年 / 11卷 / 02期

基金：

中国国家自然科学基金;

关键词：

MOSAIC-VIRUS INFECTION; TUBBY-LIKE PROTEINS; HYPERSENSITIVE RESPONSE; SIGNAL-TRANSDUCTION; MEMBRANE-PROTEIN; LEAF SENESCENCE; THALIANA NHL10; EXPRESSION; TOLERANCE; NDR1;

D O I：

10.1371/journal.pone.0148572

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

NHL (NDR1/HIN1-like) genes play crucial roles in pathogen induced plant responses to biotic stress. Here, we report the possible function of NHL6 in plant response to abscisic acid (ABA) and abiotic stress. NHL6 was highly expressed in non-germinated seeds, and its expression was strongly induced by ABA and multiple abiotic stress signals. Loss-of-function of NHL6 decreased sensitivity to ABA in the early developmental stages including seed germination and post-germination seedling growth of the nhl6 mutants. However, overexpression of NHL6 increased sensitivity to ABA, salt and osmotic stress of the transgenic plants. Further studies indicated that the increased sensitivity in the 35S:: NHL6 overexpressing plants could be a result of both ABA hypersensitivity and increased endogenous ABA accumulation under the stress conditions. It was also seen that the ABA-responsive element binding factors AREB1, AREB2 and ABF3 could regulate NHL6 expression at transcriptional level. Our results indicate that NHL6 plays an important role in the abiotic stresses-induced ABA signaling and biosynthesis, particularly during seed germination and early seedling development in Arabidopsis.

引用

页数：16

共 46 条

[1] PYRABACTIN RESISTANCE1-LIKE8 Plays an Important Role for the Regulation of Abscisic Acid Signaling in Root [J].