Immobilization of α-amylase from Bacillus circulans GRS 313 on coconut fiber

被引：33

作者：

Dey, G ^{[1
]}

Nagpal, V ^{[1
]}

Banerjee, R ^{[1
]}

机构：

[1] Indian Inst Technol, Microbial Biotechnol & Downstream Proc Lab, Agr & Food Engn Dept, Kharagpur 721302, W Bengal, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2002年 / 102卷 / 1-6期

关键词：

coconut fiber; adsorption; response surface methodology; amylase; Bacillus circulans GRS 313;

D O I：

10.1385/ABAB:102-103:1-6:303

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and inexpensive method for immobilizing a-amylase from Bacillus circulans GRS 313 on coconut fiber was developed. The immobilization conditions for highest efficiency were optimized with respect to immobilization pH of 5.5, 30degreesC, contact time of 4 h, and enzyme to support a ratio of 1:1 containing 0.12 mg/mL of protein. The catalytic properties of the immobilized enzyme were compared with that of the free enzyme. The activity of amylase adsorbed on coconut fiber was 38.7 U/g of fiber at its optimum pH of 5.7 and 48degreesC, compared with the maximum activity of 40.2 U/mL of free enzyme at the optimum pH of 4.9 and 48degreesC. The reutilization capacity of the immobilized enzyme was up to three cycles.

引用

页码：303 / 313

页数：11

共 16 条

[1] Preparation and some properties of immobilized Penicillium funiculosum 258 dextranase [J].

Abdel-Naby, MA ;

Ismail, AMS ;

Abdel-Fattah, AM ;

Abdel-Fattah, AF .

PROCESS BIOCHEMISTRY, 1999, 34 (04) :391-398

[2] Immobilization of pectin lyase from Penicillium italicum by covalent binding to nylon [J].

Alkorta, I ;

Garbisu, C ;

Llama, MJ ;

Serra, JL .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (02) :141-146

[3]

BERNATH FR, 1974, IMMOBILIZED ENZYMES

[4]

Bernfeld P., 1955, METHODS ENZYMOLOGY

[5] MULTI-FACTOR EXPERIMENTAL-DESIGNS FOR EXPLORING RESPONSE SURFACES [J].

BOX, GEP ;

HUNTER, JS .

ANNALS OF MATHEMATICAL STATISTICS, 1957, 28 (01) :195-241

[6] EFFECTS ON THE HYDROLYSIS OF NATIVE STARCH AND GLYCOGEN BY A THERMOSTABLE ALPHA-AMYLASE AFTER IMMOBILIZATION ON SOLID SUPPORTS [J].

EMNEUS, J ;

GORTON, L .

ANALYTICA CHIMICA ACTA, 1990, 234 (01) :97-106

[7]

KHURI AJ, 1987, STAT TXB MONOGRAPHS

[8]

KONDURU S, 1999, HORT SCI, V34, P1

[9]

LARTIGUE DJ, 1975, IMMOBILIZED ENZYMES

[10] STARCH CONVERSION BY SOLUBLE AND IMMOBILIZED ALPHA-AMYLASE [J].

LINKO, YY ;

SAARINEN, P ;

LINKO, M .

BIOTECHNOLOGY AND BIOENGINEERING, 1975, 17 (02) :153-165

← 1 2 →