Immobilization of Rhizopus japonicus lipase on celite and its application for enrichment of docosahexaenoic acid in soybean oil

被引：41

作者：

Khare, SK ^{[1
]}

Nakajima, M

机构：

[1] Indian Council Agr Res, Cent Inst Agr Engn, Bhopal 462038, India

[2] Minist Agr Forestry & Fisheries, Natl Food Res Inst, Tsukuba, Ibaraki 305, Japan

来源：

FOOD CHEMISTRY | 2000年 / 68卷 / 02期

关键词：

D O I：

10.1016/S0308-8146(99)00166-1

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Rhizopus japonicus lipase was immobilized on Celite, the immobilized enzyme acquired 6.34 units of transesterification activity over the native enzyme which does not show any significant transesterification activity in n-hexane. However, there was no improvement in the thermal stability of Celite-immobilized lipase. The preparation is shown to be promising for its end application in producing structured lipid. An incorporation of 25% docosahexaenoic acid (DHA) into soybean oil was observed in n-hexane media in 24 h with the immobilized lipase. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：153 / 157

页数：5

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