IGF-1 and epidermal growth factor levels in follicular fluid of women undergoing controlled ovarian hyperstimulation using the multidose GnRH-antagonist protocol or the long GnRH-agonist protocol

The intrafollicular levels of IGF-1 and epidermal growth factor (EGF) were studied in women undergoing controlled ovarian hyperstimulation using the multiclose GnRH-antagonist protocol or the long agonist protocol, in an attempt to elucidate whether GnRH-antagonists affect the levels of the two growth factors. The follicular fluid concentration of IGF-1, EGF, estradiol and progesterone were detected in 68 women undergoing ovarian hyperstimulation for intracytoplasmic sperm injection (ICSI) cycles. There were no differences in intrafollicular concentrations of EGF and IGF-1 in the two studied groups. Additionally, we found no correlation between the intrafollicular levels of IGF-1 or EGF and the ICS1 outcome. The intrafollicular levels of IGF-1 were positively correlated with those of progesterone. In conclusion, the intrafollicular levels of IGF-1 and EGF do not seem to be influenced by the stimulation protocol. The intrafollicular levels of both growth factors can not serve as prognostic markers for the ICS1 outcome.