A novel horseradish peroxidase biosensor towards the detection of dopamine: A voltammetric study

被引：46

作者：

Raghu, P. ^{[1
]}

Reddy, T. Madhusudana ^{[1
]}

Gopal, P. ^{[1
]}

Reddaiah, K. ^{[1
]}

Sreedhar, N. Y. ^{[1
]}

机构：

[1] Sri Venkateswara Univ, Dept Chem, SVU Coll Sci, Electrochem Res Lab, Tirupati 517502, Andhra Pradesh, India

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2014年 / 57卷

关键词：

Carbon paste electrode; Horseradish peroxidase; Glycine; Multiwalled carbon nanotubes; Silica sol-gel; Dopamine; GLASSY-CARBON ELECTRODE; GEL IMMOBILIZED BIOSENSOR; SOL-GEL; HYDROGEN-PEROXIDE; AMPEROMETRIC BIOSENSOR; ASCORBIC-ACID; PHARMACEUTICAL FORMULATIONS; ELECTROCHEMICAL DETECTION; SWEEP VOLTAMMETRY; COATED ELECTRODE;

D O I：

10.1016/j.enzmictec.2014.01.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A polymerized film of glycine (Gly) was prepared on the surface of carbon paste electrode (CPE) through the cyclic voltammetry (CV) technique. A novel biosensor for the determination of dopamine (DA) has been constructed based on horseradish peroxidase (HRP) and multiwalled carbon nanotubes (MWCNTs) immobilizing on Poly (Gly)/CPE through silica sol-gel (SiSG) entrapment. CV measurements were employed in order to understand the feasibility of poly (Gly) as an electron carrier between the immobilized peroxidase and the surface of CPE. By using differential pulse voltammetry (DPV) the calibration curves of DA was obtained in the range of 15-865 mu M. The limit of detection (LOD) and limit of quantification (LOQ) of DA was found to be 6 x 10(-7) M and 2 x 10(-6) M respectively. The apparent Michaelis-Menten constant (K-m(app)) was found to be 0.5 mM and illustrated that the good biological activity of the fixed enzyme. Electrochemical impedance spectroscopy (EIS) results confirmed the rapid electron transfer and also the immobilization of enzyme on the electrode surface. The biosensor showed high sensitivity, selectivity and reproducibility. This method has been used to determine DA in the presence of various interferences and in clinical preparations. (c) 2014 Elsevier Inc. All rights reserved.

引用

页码：8 / 15

页数：8

共 57 条

[1] Nanotubes from carbon [J].