A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors

被引：134

作者：

Bouyer, Charlene ^{[1
,2
,3
]}

Chen, Pu ^{[1
]}

Gueven, Sinan ^{[1
]}

Demirtas, Tugrul Tolga ^{[1
]}

Nieland, Thomas J. F. ^{[1
]}

Padilla, Frederic ^{[2
,3
]}

Demirci, Utkan ^{[1
,4
]}

机构：

[1] Stanford Univ, Bioacoust MEMS Med BAMM Lab, Dept Radiol, Canary Ctr Early Canc Detect,Sch Med, Palo Alto, CA 94304 USA

[2] Univ Lyon, LabTau, INSERM, U1032, F-69003 Lyon, France

[3] Univ Lyon, LabEx DEVweCAN, F-69003 Lyon, France

[4] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

来源：

ADVANCED MATERIALS | 2016年 / 28卷 / 01期

基金：

美国国家科学基金会;

关键词：

TISSUE; ASTROCYTES; CORTEX; FIBRIN;

D O I：

10.1002/adma.201503916

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A bio-acoustic levitational assembly method for engineering of multilayered, 3D brainlike constructs is presented. Acoustic radiation forces are used to levitate neuro-progenitors derived from human embryonic stem cells in 3D multilayered fibrin tissue constructs. The neuro-progenitor cells are subsequently differentiated in neural cells, resulting in a 3D neuronal construct with inter and intralayer neurite elongations.

引用

页码：161 / +

页数：8

共 27 条

[1] Fibrin: A versatile scaffold for tissue engineering applications [J].

Ahmed, Tamer A. E. ;

Dare, Emma V. ;

Hincke, Max .

TISSUE ENGINEERING PART B-REVIEWS, 2008, 14 (02) :199-215

[2] Tissue Engineering at the Micro-Scale [J].

Bhatia, Sangeeta N. ;

Chen, Christopher S. .

BIOMEDICAL MICRODEVICES, 1999, 2 (02) :131-144

[3] Acoustofluidics 2: Perturbation theory and ultrasound resonance modes [J].

Bruus, Henrik .

LAB ON A CHIP, 2012, 12 (01) :20-28

[4] Synaptic transmission in the striatum: from plasticity to neurodegeneration [J].

Calabresi, P ;

Centonze, D ;

Gubellini, P ;

Marfia, GA ;

Pisani, A ;

Sancesario, G ;

Bernardi, G .

PROGRESS IN NEUROBIOLOGY, 2000, 61 (03) :231-265

[5] Brain slices as models for neurodegenerative disease and screening platforms to identify novel therapeutics [J].

Cho, Seongeun ;

Wood, Andrew ;

Bowby, Mark R. .

CURRENT NEUROPHARMACOLOGY, 2007, 5 (01) :19-33

[6] The Human Brain in a Dish: The Promise of iPSC-Derived Neurons [J].

Dolmetsch, Ricardo ;

Geschwind, Daniel H. .

CELL, 2011, 145 (06) :831-834

[7] Matrix elasticity directs stem cell lineage specification [J].

Engler, Adam J. ;

Sen, Shamik ;

Sweeney, H. Lee ;

Discher, Dennis E. .

CELL, 2006, 126 (04) :677-689

[8] Early involvement of the cerebral cortex in Parkinson's disease: Convergence of multiple metabolic defects [J].

Ferrer, Isidre .

PROGRESS IN NEUROBIOLOGY, 2009, 88 (02) :89-103

[9] Measuring the thickness of the human cerebral cortex from magnetic resonance images [J].

Fischl, B ;

Dale, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (20) :11050-11055

[10] The use of the hippocampal slice preparation in the study of Alzheimer's disease [J].

Fitzjohn, Stephen M. ;

Doherty, Andrew J. ;

Collingridge, Graham L. .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2008, 585 (01) :50-59

← 1 2 3 →