Acute effect of growth hormone to induce peripheral insulin resistance is independent of FFA and insulin levels in rats

被引:13
作者
Kim, JK [1 ]
Choi, CS [1 ]
Youn, JH [1 ]
机构
[1] Univ So Calif, Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90089 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1999年 / 277卷 / 04期
关键词
glycolysis; glycogen synthesis; skeletal muscle; glucose; 6-phosphate; growth hormone; free fatty acid;
D O I
10.1152/ajpendo.1999.277.4.E742
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Acute effect of growth hormone to induce peripheral insulin resistance is independent of FFA and insulin levels in rats. To examine whether growth hormone (GH) induces peripheral insulin resistance by altering plasma free fatty acid (FFA) or insulin levels, the effects of GH infusion on insulin-stimulated glucose fluxes were studied in conscious rats under two protocols. In study 1, either saline (n = 7) or human recombinant GH (21 mu g . kg(-1) . h(-1); n = 8) was infused for 300 min, and insulin-stimulated glucose fluxes were estimated during the final 150-min period of hyperinsulinemic euglycemic clamps. In study 2, hyperinsulinemic euglycemic clamps were first conducted for 150 min (to raise plasma insulin and suppress FFA levels), and saline or GH (n = 7 for each) was subsequently infused for the following 300-min clamp period. In study 1, GH infusion in the basal state did not significantly alter plasma FFA or insulin levels. In contrast, GH infusion decreased insulin-stimulated glucose uptake, glycolysis, and glycogen synthesis by 32, 27, and 40%, respectively (P < 0.05). In study 2, GH infusion during hyperinsulinemic euglycemic clamps did not alter plasma FFA or insulin levels (P > 0.05). GH infusion had no effect on insulin-stimulated glucose uptake during the initial 150 min but eventually decreased insulin-stimulated glucose uptake by 37% (P < 0.05), similar to the results in study 1. These data indicate that GH induces peripheral insulin resistance independent of plasma FFA and insulin levels. The induction of insulin resistance was preceded by suppression of glycogen synthesis, consistent with the hypothesis that metabolic impairment precedes and causes development of peripheral insulin resistance.
引用
收藏
页码:E742 / E749
页数:8
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