iTRAQ-Based Proteomic Analysis of Dentate Gyrus in Temporal Lobe Epilepsy With Hippocampal Sclerosis

被引:6
作者
Xiao, Wenbiao [1 ]
Yang, Zhiquan [2 ]
Yan, Xiaoxin [3 ]
Feng, Li [1 ]
Long, Lili [1 ]
Tu, Tian [3 ]
Deng, Na [1 ]
Chen, Wenjuan [4 ]
Xiao, Bo [1 ]
Long, Hongyu [1 ]
Zeng, Yi [5 ]
机构
[1] Cent South Univ, Xiangya Hosp, Dept Neurol, Changsha, Peoples R China
[2] Cent South Univ, Xiangya Hosp, Dept Neurosurg, Changsha, Peoples R China
[3] Cent South Univ, Xiangya Sch Med, Dept Anat & Neurobiol, Changsha, Peoples R China
[4] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Psychiat, Sch Med, Hangzhou, Peoples R China
[5] Cent South Univ, Xiangya Hosp 2, Dept Geriatr, Changsha, Peoples R China
基金
中国国家自然科学基金;
关键词
temporal lobe epilepsy; hippocampal sclerosis; dentate gyrus; quantitative proteomics; iTRAQ;
D O I
10.3389/fneur.2020.626013
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Temporal lobe epilepsy (TLE) is the most frequent type of focal epilepsy in adults, typically resistant to pharmacological treatment, and mostly presents with cognitive impairment and psychiatric comorbidities. The most common neuropathological hallmark in TLE patients is hippocampal sclerosis (HS). However, the underlying molecular mechanisms involved remain poorly characterized. The dentate gyrus (DG), one specific hippocampal subarea, structural and functional changes imply a key involvement of the DG in the development of TLE. In this study, a isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic technique was performed for the analysis of hippocampal DG obtained from patients with TLE-HS compared to control samples obtained from autopsy. Our proteomic data identified 5,583 proteins, of which 82 proteins were upregulated and 90 proteins were downregulated. Bioinformatics analysis indicated that differentially expressed proteins were enriched in "synaptic vesicle," "mitochondrion," "cell-cell adhesion," "regulation of synaptic plasticity," "ATP binding," and "oxidative phosphorylation." Protein-protein interaction network analysis found a pivotal module of 10 proteins that were related to "oxidative phosphorylation." This study has investigated proteomic alterations in the DG region of TLE-HS patients, and paved the way for the better understanding of epileptogenesis mechanisms and future therapeutic intervention.
引用
收藏
页数:10
相关论文
共 49 条
[1]   Hippocampal CA3 Transcriptome Signature Correlates with Initial Precipitating Injury in Refractory Mesial Temporal Lobe Epilepsy [J].
Bando, Silvia Y. ;
Alegro, Maryana C. ;
Amaro, Edson, Jr. ;
Silva, Alexandre V. ;
Castro, Luiz H. M. ;
Wen, Hung-Tzu ;
Lima, Leandro de A. ;
Brentani, Helena ;
Moreira-Filho, Carlos Alberto .
PLOS ONE, 2011, 6 (10)
[2]   Chronic systemic pesticide exposure reproduces features of Parkinson's disease [J].
Betarbet, R ;
Sherer, TB ;
MacKenzie, G ;
Garcia-Osuna, M ;
Panov, AV ;
Greenamyre, JT .
NATURE NEUROSCIENCE, 2000, 3 (12) :1301-1306
[3]   Neurotoxic in vivo models of Parkinson's disease: recent advances [J].
Bjorklund, Anders ;
Cenci, M. Angela .
RECENT ADVANCES IN PARKINSONS DISEASE: TRANSLATIONAL AND CLINICAL RESEARCH, 2010, 184 :17-33
[4]   International consensus classification of hippocampal sclerosis in temporal lobe epilepsy: A Task Force report from the ILAE Commission on Diagnostic Methods [J].
Bluemcke, Ingmar ;
Thom, Maria ;
Aronica, Eleonora ;
Armstrong, Dawna D. ;
Bartolomei, Fabrice ;
Bernasconi, Andrea ;
Bernasconi, Neda ;
Bien, Christian G. ;
Cendes, Fernando ;
Coras, Roland ;
Cross, J. Helen ;
Jacques, Thomas S. ;
Kahane, Philippe ;
Mathern, Gary W. ;
Miyata, Haijme ;
Moshe, Solomon L. ;
Oz, Buge ;
Oezkara, Cigdem ;
Perucca, Emilio ;
Sisodiya, Sanjay ;
Wiebe, Samuel ;
Spreafico, Roberto .
EPILEPSIA, 2013, 54 (07) :1315-1329
[5]   RNAseq analysis of hippocampal microglia after kainic acid-induced seizures [J].
Bosco, Dale B. ;
Zheng, Jiaying ;
Xu, Zhiyan ;
Peng, Jiyun ;
Eyo, Ukpong B. ;
Tang, Ke ;
Yan, Cheng ;
Huang, Jun ;
Feng, Lijie ;
Wu, Gongxiong ;
Richardson, Jason R. ;
Wang, Hui ;
Wu, Long-Jun .
MOLECULAR BRAIN, 2018, 11
[6]   Regulators of synaptic transmission: Roles in the pathogenesis and treatment of epilepsy [J].
Casillas-Espinosa, Pablo M. ;
Powell, Kim L. ;
O'Brien, Terence J. .
EPILEPSIA, 2012, 53 :41-58
[7]  
Cuperfain Ari B, 2018, Mol Neuropsychiatry, V4, P52, DOI 10.1159/000488031
[8]   Mitochondrial dysfunction in epilepsy [J].
Folbergrova, Jaroslava ;
Kunz, Wolfram S. .
MITOCHONDRION, 2012, 12 (01) :35-40
[9]   Differential gene expression in dentate granule cells in mesial temporal lobe epilepsy with and without hippocampal sclerosis [J].
Griffin, Nicole G. ;
Wang, Yu ;
Hulette, Christine M. ;
Halvorsen, Matt ;
Cronin, Kenneth D. ;
Walley, Nicole M. ;
Haglund, Michael M. ;
Radtke, Rodney A. ;
Skene, J. H. Pate ;
Sinha, Saurabh R. ;
Heinzen, Erin L. .
EPILEPSIA, 2016, 57 (03) :376-385
[10]   Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources [J].
Huang, Da Wei ;
Sherman, Brad T. ;
Lempicki, Richard A. .
NATURE PROTOCOLS, 2009, 4 (01) :44-57