Colorimetric Sensing by Using Allosteric-DNAzyme-Coupled Rolling Circle Amplification and a Peptide Nucleic Acid-Organic Dye Probe

被引：122
作者：
Ali, M. Monsur
Li, Yingfu [1 ]
机构：
[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 19期
关键词：
biosensors; colorimetry; DNAzymes; peptide nucleic acids; rolling circle amplification; IN-VITRO SELECTION; PNA-DNA DUPLEXES; SECONDARY STRUCTURE; PROTEIN-DETECTION; CYANINE DYE; APTAMERS; DEOXYRIBOZYME; OLIGONUCLEOTIDES; MOLECULES; ENZYMES;
D O I：
10.1002/anie.200805966
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Target detection by the naked eye: The action of an RNA-cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single-stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex-binding dye, the color of the dye changed from blue to purple. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3512 / 3515
页数：4
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