WTIP interacts with ASXL2 and blocks ASXL2-mediated activation of retinoic acid signaling

被引：9
作者：
Khan, Farida F. [1 ]
Li, Yanyang [1 ]
Balyan, Arjun [1 ]
Wang, Q. Tian [1 ]
机构：
[1] Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 451卷 / 01期
关键词：
ASXL2; WTIP; Retinoic acid signaling; Epicardium; Heart development; ADDITIONAL-SEX-COMBS; BOHRING-OPITZ SYNDROME; GENE IN-SILICO; RECIPROCAL REGULATION; DROSOPHILA ENHANCER; MURINE HOMOLOG; LIM DOMAIN; MUTATIONS; POLYCOMB; WT1;
D O I：
10.1016/j.bbrc.2014.07.080
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Asx-like (ASXL) family proteins are chromatin factors that play dual roles in transcriptional activation and repression. ASXL2 is highly expressed in the heart and is required for proper heart development and function. Here, we identify a novel ASXL2-binding partner, the LIM domain-containing protein WTIP. Genetic and biochemical assays show a direct interaction between ASXL2 and WTIP. In HeLa cells, ASXL2 enhances retinoic acid-dependent luciferase activity, while WTIP represses it. Furthermore, WTIP blocks ASXL2's stimulatory effect on transcription. In addition, we found that ASXL2 and WTIP are expressed in mouse embryonic epicardial cells, a tissue that is regulated by retinoic acid signaling. Together, these results implicate ASXL2 and WTIP in regulation of retinoic acid signaling during heart development. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：101 / 106
页数：6
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