Production of bone marrow, liver, thymus (BLT) humanized mice on the C57BL/6 Rag2-/-γc-/-CD47-/- background

被引：21

作者：

Lavender, Kerry J. ^{[1
]}

Messer, Ronald J. ^{[1
]}

Race, Brent ^{[1
]}

Hasenkrug, Kim J. ^{[1
]}

机构：

[1] NIAID, Lab Persistent Viral Dis, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2014年 / 407卷

关键词：

ELT humanized mice; C57BL/6 Rag2(-/-)gamma(-/-)(c)CD47(-/-); Hematopoiesis; HEMATOPOIETIC STEM-CELLS; RECONSTITUTION; RAG2;

D O I：

10.1016/j.jim.2014.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

C57BL/6 Rag2(-/-)gamma(-/-)(c)CD47(-/-) triple-knockout mice engrafted with fetal human bone marrow, liver, thymus (TKO-BLT) not only develop high levels of multi-lineage hematopoiesis but also organized lymphoid tissues including mesenteric lymph nodes, splenic follicles and gut-associated lymphoid tissues of human origin. A unique advantage of these mice is that they sustain human cell and tissue engraftment long-term without the development of graft versus host disease. Thus they can be used for long-term studies not previously feasible with other models. The production of TKO-BLT mice to obtain healthy mice with high level reconstitution of human cells and tissues requires specialized methods that are presented in detail. Published by Elsevier B.V.

引用

页码：127 / 134

页数：8

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