Honokiol suppresses TNF-α-induced migration and matrix metalloproteinase expression by blocking NF-κB activation via the ERK signaling pathway in rat aortic smooth muscle cells

Honokiol, a small-molecule polyphenol derived and isolated from the Chinese medicinal herb Magnolia officinalis, has been shown to possess a wide range of pharmacological activities. In the present study, we aimed to investigate the effects of honokiol on tumor necrosis factor-a (TNF-alpha)-induced migration in rat aortic smooth muscle cells (RASMCs). We found that honokiol inhibited TNF-a-induced RASMC proliferation and migration in a dose-dependent manner. At the molecular level, pretreatment with honokiol blocked TNF-alpha-induced protein expression of matrix metalloproteinase (MMP)-2 and MMP-9, nuclear factor (NF)-kappa B activation, and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. Moreover, NF-kappa B inhibitor (BAY 11-7028) and ERK inhibitor (U0126) also mimicked the inhibitory effects of honokiol in TNF-alpha-treated RASMCs. In conclusion, these results indicate that honokiol suppresses TNF-alpha-induced migration and MMP expression by blocking NF-kappa B activation via the ERK signaling pathway in RASMCs. Our findings support honokiol as a promising novel agent for the prevention and treatment of atherosclerosis. (C) 2013 Elsevier GmbH. All rights reserved.