Prolonged Nitric Oxide Exposure Enhances Anoikis Resistance and Migration through Epithelial-Mesenchymal Transition and Caveolin-1 Upregulation

被引：21

作者：

Chanvorachote, Pithi ^{[1
]}

Pongrakhananon, Varisa

Chunhacha, Preedakorn

机构：

[1] Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Physiol & Pharmacol, Bangkok 10330, Thailand

来源：

BIOMED RESEARCH INTERNATIONAL | 2014年 / 2014卷

关键词：

POOR-PROGNOSIS; CELL-MIGRATION; METASTASIS; EXPRESSION; APOPTOSIS; INHIBITION; INVASION; SURVIVAL; GROWTH; EMT;

D O I：

10.1155/2014/941359

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nitric oxide (NO) in tumor microenvironment may have a significant impact on metastatic behaviors of cancer. Noncytotoxic doses of NO enhanced anoikis resistance and migration in lung cancer H23 cells via an increase in lamellipodia, epithelial-mesenchymal transition (EMT) markers including vimentin and snail, and caveolin-1 (Cav-1). However, the induction of EMT was found in Cav-1-knock down cells treated with NO, suggesting that EMT was through Cav-1-independent pathway. These effects of NO were consistently observed in other lung cancer cells including H292 and H460 cells. These findings highlight the novel role of NO on EMT and metastatic behaviors of cancer cells.

引用

页数：10

共 32 条

[1] Actin-dependent lamellipodia formation and microtubule-dependent tail retraction control-directed cell migration [J].

Ballestrem, C ;

Wehrle-Haller, B ;

Hinz, B ;

Imhof, BA .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (09) :2999-3012

[2] Mechanisms of nitric oxide-mediated inhibition of EMT in cancer Inhibition of the metastasis-inducer snail and induction of the metastasis-suppressor RKIP [J].

Baritaki, Stavroula ;

Huerta-Yepez, Sara ;

Sahakyan, Anna ;

Karagiannides, Iordanis ;

Bakirtzi, Kyriaki ;

Jazirehi, Ali ;

Bonavida, Benjamin .

CELL CYCLE, 2010, 9 (24) :4931-4940

[3] Nitric oxide negatively regulates Fas CD95-induced apoptosis through inhibition of ubiquitin-proteasome-mediated degradation of FLICE inhibitory protein [J].

Chanvorachote, P ;

Nimmannit, U ;

Wang, LY ;

Stehlik, C ;

Lu, B ;

Azad, N ;

Rojanasakul, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (51) :42044-42050

[4] Nitric oxide regulates cell sensitivity to cisplatin-induced apoptosis through S-nitrosylation and inhibition of Bcl-2 ubiquitination [J].

Chanvorachote, Pithi ;

Nimmannit, Ubonthip ;

Stehlik, Christian ;

Wang, Liying ;

Jiang, Bing-Hua ;

Ongpipatanakul, Boonsri ;

Rojanasakul, Yon .

CANCER RESEARCH, 2006, 66 (12) :6353-6360

[5] Anoikis: a potential target to prevent lung cancer metastasis? [J].

Chanvorachote, Pithi ;

Chunhacha, Preedakorn ;

Pongrakhananon, Varisa .

LUNG CANCER MANAGEMENT, 2013, 2 (03) :169-171

[6] Nitric Oxide Regulates Lung Carcinoma Cell Anoikis through Inhibition of Ubiquitin-Proteasomal Degradation of Caveolin-1 [J].

Chanvorachote, Pithi ;

Nimmannit, Ubonthip ;

Lu, Yongju ;

Talbott, Siera ;

Jiang, Bing-Hua ;

Rojanasakul, Yon .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (41) :28476-28484

[7] Epithelial-mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion-derived human lung cancer cells [J].

Chunhacha, Preedakorn ;

Sriuranpong, Virote ;

Chanvorachote, Pithi .

ONCOLOGY LETTERS, 2013, 5 (03) :1043-1047

[8]

Chunhacha Preedakorn, 2012, Int J Physiol Pathophysiol Pharmacol, V4, P149

[9] Caveolin-1 regulates Mcl-1 stability and anoikis in lung carcinoma cells [J].

Chunhacha, Preedakorn ;

Pongrakhananon, Varisa ;

Rojanasakul, Yon ;

Chanvorachote, Pithi .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2012, 302 (09) :C1284-C1292

[10] Collective cell migration in morphogenesis, regeneration and cancer [J].

Friedl, Peter ;

Gilmour, Darren .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (07) :445-457

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