Prolonged Nitric Oxide Exposure Enhances Anoikis Resistance and Migration through Epithelial-Mesenchymal Transition and Caveolin-1 Upregulation

被引：21
作者：
Chanvorachote, Pithi [1 ]
Pongrakhananon, Varisa
Chunhacha, Preedakorn
机构：
[1] Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Physiol & Pharmacol, Bangkok 10330, Thailand
来源：
BIOMED RESEARCH INTERNATIONAL | 2014年 / 2014卷
关键词：
POOR-PROGNOSIS; CELL-MIGRATION; METASTASIS; EXPRESSION; APOPTOSIS; INHIBITION; INVASION; SURVIVAL; GROWTH; EMT;
D O I：
10.1155/2014/941359
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Nitric oxide (NO) in tumor microenvironment may have a significant impact on metastatic behaviors of cancer. Noncytotoxic doses of NO enhanced anoikis resistance and migration in lung cancer H23 cells via an increase in lamellipodia, epithelial-mesenchymal transition (EMT) markers including vimentin and snail, and caveolin-1 (Cav-1). However, the induction of EMT was found in Cav-1-knock down cells treated with NO, suggesting that EMT was through Cav-1-independent pathway. These effects of NO were consistently observed in other lung cancer cells including H292 and H460 cells. These findings highlight the novel role of NO on EMT and metastatic behaviors of cancer cells.
引用
收藏
页数：10
相关论文
共 32 条
  • [1] Actin-dependent lamellipodia formation and microtubule-dependent tail retraction control-directed cell migration
    Ballestrem, C
    Wehrle-Haller, B
    Hinz, B
    Imhof, BA
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (09) : 2999 - 3012
  • [2] Mechanisms of nitric oxide-mediated inhibition of EMT in cancer Inhibition of the metastasis-inducer snail and induction of the metastasis-suppressor RKIP
    Baritaki, Stavroula
    Huerta-Yepez, Sara
    Sahakyan, Anna
    Karagiannides, Iordanis
    Bakirtzi, Kyriaki
    Jazirehi, Ali
    Bonavida, Benjamin
    [J]. CELL CYCLE, 2010, 9 (24) : 4931 - 4940
  • [3] Nitric oxide negatively regulates Fas CD95-induced apoptosis through inhibition of ubiquitin-proteasome-mediated degradation of FLICE inhibitory protein
    Chanvorachote, P
    Nimmannit, U
    Wang, LY
    Stehlik, C
    Lu, B
    Azad, N
    Rojanasakul, Y
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (51) : 42044 - 42050
  • [4] Nitric oxide regulates cell sensitivity to cisplatin-induced apoptosis through S-nitrosylation and inhibition of Bcl-2 ubiquitination
    Chanvorachote, Pithi
    Nimmannit, Ubonthip
    Stehlik, Christian
    Wang, Liying
    Jiang, Bing-Hua
    Ongpipatanakul, Boonsri
    Rojanasakul, Yon
    [J]. CANCER RESEARCH, 2006, 66 (12) : 6353 - 6360
  • [5] Anoikis: a potential target to prevent lung cancer metastasis?
    Chanvorachote, Pithi
    Chunhacha, Preedakorn
    Pongrakhananon, Varisa
    [J]. LUNG CANCER MANAGEMENT, 2013, 2 (03) : 169 - 171
  • [6] Nitric Oxide Regulates Lung Carcinoma Cell Anoikis through Inhibition of Ubiquitin-Proteasomal Degradation of Caveolin-1
    Chanvorachote, Pithi
    Nimmannit, Ubonthip
    Lu, Yongju
    Talbott, Siera
    Jiang, Bing-Hua
    Rojanasakul, Yon
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (41) : 28476 - 28484
  • [7] Epithelial-mesenchymal transition mediates anoikis resistance and enhances invasion in pleural effusion-derived human lung cancer cells
    Chunhacha, Preedakorn
    Sriuranpong, Virote
    Chanvorachote, Pithi
    [J]. ONCOLOGY LETTERS, 2013, 5 (03) : 1043 - 1047
  • [8] Chunhacha Preedakorn, 2012, Int J Physiol Pathophysiol Pharmacol, V4, P149
  • [9] Caveolin-1 regulates Mcl-1 stability and anoikis in lung carcinoma cells
    Chunhacha, Preedakorn
    Pongrakhananon, Varisa
    Rojanasakul, Yon
    Chanvorachote, Pithi
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2012, 302 (09): : C1284 - C1292
  • [10] Collective cell migration in morphogenesis, regeneration and cancer
    Friedl, Peter
    Gilmour, Darren
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (07) : 445 - 457
1234