Exploring polar headgroup interactions between sphingomyelin and ceramide with infrared spectroscopy

被引:21
|
作者
de la Arada, Igor [1 ,2 ]
Gonzalez-Ramirez, Emilio J. [1 ,2 ]
Alonso, Alicia [1 ,2 ]
Goni, Felix M. [1 ,2 ]
Arrondo, Jose-Luis R. [1 ,2 ]
机构
[1] Univ Basque Country, UPV EHU, Inst Biofis CSIC, Leioa 48940, Spain
[2] Univ Basque Country, Dept Bioquim, Leioa 48940, Spain
关键词
LIPID INTERACTIONS; PHASE; SPHINGOLIPIDS; MEMBRANES; DOMAINS; CHOLESTEROL; COEXISTENCE; MONOLAYERS; PROTEINS;
D O I
10.1038/s41598-020-74781-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Ceramide is a major actor in the sphingolipid signaling pathway elicited by various kinds of cell stress. Under those conditions ceramide (Cer) is produced in the plasma membrane as a product of sphingomyelin (SM) hydrolysis, and this may lead to apoptosis. Thus, SM and Cer coexist in the membrane for some time, and they are known to separate laterally from the (more abundant) glycerolipids, giving rise to highly rigid domains or platforms. The properties of these domains/platforms are rather well understood, but the underlying SM:Cer molecular interactions have not been explored in detail. Infrared (IR) spectroscopy is a powerful analytical technique that provides information on all the chemical groupings in a molecule, and that can be applied to membranes and lipid bilayers in aqueous media. IR spectra can be conveniently retrieved as a function of temperature, thus revealing the thermotropic transitions of SM and its mixtures with Cer. Four regions of the IR spectrum of these sphingolipids have been examined, two of them dominated by the hydrophobic regions in the molecules, namely the C-H stretching vibrations (2800-3000 cm(-1)), and the CH2 scissoring vibrations (1455-1485 cm(-1)), and two others arising from chemical groups at the lipid-water interface, the sphingolipid amide I band (1600-1680 cm(-1)), and the phosphate vibrations in the 1000-1110 cm(-1) region. The latter two regions have been rarely studied in the past. The IR data from the hydrophobic components show a gel (or ripple)-fluid transition of SM at 40 degrees C, that is shifted up to about 70 degrees C when Cer is added to the bilayers, in agreement with previous studies using a variety of techniques. IR information concerning the polar parts is more interesting. The amide I (carbonyl) band of pure SM exhibits a maximum at 1638 cm(-1) at room temperature, and its position is shifted by about 10 cm(-1) in the presence of Cer. Cer causes also a change in the overall band shape, but no signs of band splitting are seen, suggesting that SM and Cer carbonyl groups are interacting tightly, presumably through H-bonds. The 1086 cm(-1) band, corresponding to PO2- vibrations, appears more stable in SM than in DPPC, and it is further stabilized by Cer, again suggesting an important role of H-bonds in the formation of SM:Cer clusters. Thus, SM and Cer can interact through their polar headgroups, in a way that is not accessible to other lipid classes.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] Exploring polar headgroup interactions between sphingomyelin and ceramide with infrared spectroscopy
    Igor de la Arada
    Emilio J. González-Ramírez
    Alicia Alonso
    Félix M. Goñi
    José-Luis R. Arrondo
    Scientific Reports, 10
  • [2] Effects of Sphingomyelin Headgroup Size on Interactions with Ceramide
    Artetxe, Ibai
    Sergelius, Christian
    Kurita, Mayuko
    Yamaguchi, Shou
    Katsumura, Shigeo
    Slotte, J. Peter
    Maula, Terhi
    BIOPHYSICAL JOURNAL, 2013, 104 (03) : 604 - 612
  • [4] Cholesterol interactions with ceramide and sphingomyelin
    Garcia-Arribas, Aritz B.
    Alonso, Alicia
    Goni, Felix M.
    CHEMISTRY AND PHYSICS OF LIPIDS, 2016, 199 : 26 - 34
  • [5] Effects of metal ions on the conformation of polar headgroup of sphingomyelin bilayer
    袁春波
    赵大庆
    赵冰
    倪嘉缵
    黄芬
    ChineseScienceBulletin, 1995, (10) : 820 - 823
  • [6] Solvation and Hydration of the Ceramide Headgroup in a Non-Polar Solution
    Gillams, Richard J.
    Busto, Jon V.
    Busch, Sebastian
    Goni, Felix M.
    Lorenz, Christian D.
    McLain, Sylvia E.
    JOURNAL OF PHYSICAL CHEMISTRY B, 2015, 119 (01): : 128 - 139
  • [7] EFFECTS OF METAL-IONS ON THE CONFORMATION OF POLAR HEADGROUP OF SPHINGOMYELIN BILAYER
    YUAN, CB
    ZHAO, DQ
    ZHAO, B
    NI, JZ
    HUANG, F
    CHINESE SCIENCE BULLETIN, 1995, 40 (10): : 820 - 823
  • [8] Effect of Sphingomyelin Headgroup Size on Molecular Properties and Interactions with Cholesterol
    Bjorkbom, Anders
    Rog, Tomasz
    Kaszuba, Karol
    Kurita, Mayuko
    Yamaguchi, Shou
    Lonnfors, Max
    Nyholm, Thomas K. M.
    Vattulainen, Ilpo
    Katsumura, Shigeo
    Slotte, J. Peter
    BIOPHYSICAL JOURNAL, 2010, 99 (10) : 3300 - 3308
  • [9] Intramolecular interactions in the polar headgroup of sphingosine: serinol
    Loru, Donatella
    Pena, Isabel
    Alonso, Jose L.
    Sanz, M. Eugenia
    CHEMICAL COMMUNICATIONS, 2016, 52 (18) : 3615 - 3618
  • [10] Interactions of Ceramide and Sphingomyelin Quantified in Mixtures with an Unsaturated Phosphatidylcholine
    Castro, Bruno M.
    de Almeida, Rodrigo F. M.
    Silva, Liana C.
    Prieto, Manuel
    BIOPHYSICAL JOURNAL, 2009, 96 (03) : 355A - 356A