Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro

被引:9
|
作者
Zhu, Xiaoling [1 ]
Cai, Limin [2 ]
Meng, Qinggang [3 ]
Jin, Xiaoming [4 ]
机构
[1] First Hosp Harbin, Dept Orthopaed Surg, Harbin 150010, Heilongjiang, Peoples R China
[2] Harbin Med Univ, Dept Dermatol, Affiliated Hosp 1, Harbin 150089, Heilongjiang, Peoples R China
[3] First Hosp Harbin, Dept Orthopaed Surg, Harbin 150010, Heilongjiang, Peoples R China
[4] Harbin Med Univ, Dept Pathol, Basic Med Sci Coll, Harbin 150081, Heilongjiang, Peoples R China
关键词
gelsolin; proliferation; invasion; clear cell renal cell carcinoma; E-CADHERIN; CANCER; EXPRESSION; SUBSTRATE;
D O I
10.3892/mmr.2015.4313
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The aim of the present study was to investigate the effect of gelsolin (GSN) on the proliferation and invasion of the 786-0 clear cell renal cell carcinoma (ccRCC) cell line in vitro. A GSN overexpression lentiviral vector was constructed and transfected into 786-0 ccRCC cells in vitro. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was conducted to detect the effect of GSN on the proliferation and adhesion ability of the 786-0 ccRCC cells, and a Transwell invasion assay was used to determine the effect of GSN on the invasion of 786-0 ccRCC cells. In addition, the expression levels of invasion-associated proteins, matrix metalloproteinase (MMP)2, MMP9 and E-cadherin were analyzed by ELISA and western blotting. The MTT assay demonstrated a significantly lower optical density value for the 786-0/GSN cells compared with that of the 786-0/green fluorescent protein (GFP) and 786-0 cells following 24- and 48-h culture (P<0.05). The mean penetration rate of the 786-0/GSN cells was significantly lower than that of the 786-0/GFP and 786-0 cells (P<0.05) according to the Transwell invasion assay. The expression levels of MMP2 and MMP9 were significantly decreased in the 786-0/GSN cells, when compared with the 786-0/GFP and 786-0 cells following a 48-h transfection, according to ELISA (P<0.001). Furthermore, in the 786-0/GSN cells, the expression levels of MMP2 and MMP9 were markedly decreased, while the expression of E-cadherin was markedly increased. Thus, the overexpression of GSN may inhibit the proliferation, adhesion ability and invasion of 786-0 ccRCC cells. Additionally, GSN downregulated the expression of MMP2 and MMP9, and upregulated the expression of E-cadherin in the 786-0 ccRCC cells, which may have suppressed the invasion ability of the 786-0 ccRCC cells.
引用
收藏
页码:6887 / 6894
页数:8
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