Expression, Purification and Molecular Structure Modeling of Thioredoxin (Trx) and Thioredoxin Reductase (TrxR) from Acidithiobacillus ferrooxidans

被引：5
作者：
Wang, Yiping [1 ]
Zhang, Xiaojian [1 ]
Liu, Qing [1 ]
Ai, Chenbing [1 ]
Mo, Hongyu [1 ]
Zeng, Jia [1 ,2 ]
机构：
[1] Cent S Univ, Sch Resources Proc & Bioengn, Dept Bioengn, Changsha 410083, Hunan, Peoples R China
[2] Hunan Univ, Ctr Biomed Engn, Changsha 410082, Hunan, Peoples R China
来源：
CURRENT MICROBIOLOGY | 2009年 / 59卷 / 01期
关键词：
Acidithiobacillus ferrooxidans; Molecular structure modeling; Mutation; Thioredoxin; Thioredoxin reductase; ESCHERICHIA-COLI THIOREDOXIN; DISULFIDE; MUTATIONS; OXIDATION; MECHANISM;
D O I：
10.1007/s00284-009-9390-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The thioredoxin system consists of thioredoxin (Trx), thioredoxin reductase (TrxR) and NADPH, which plays several key roles in maintaining the redox environment of the cell. In Acidithiobacillus ferrooxidans, thioredoxin system may play important functions in the activity regulation of periplasmic proteins and energy metabolism. Here, we cloned thioredoxin (trx) and thioredoxin reductase (trxR) genes from Acidithiobacillus ferrooxidans, and expressed the genes in Escherichia coli. His-Trx and His-TrxR were purified to homogeneity with one-step Ni-NTA affinity column chromatography. Site-directed mutagenesis results confirmed that Cys33, Cys36 of thioredoxin, and Cys142, Cys145 of thioredoxin reductase were active-site residues.
引用
收藏
页码：35 / 41
页数：7
相关论文
共 50 条
  • [31] Molecular cloning, expression, purification and characterization of thioredoxin from Antarctic sea-ice bacteria Pseudoalteromonas sp AN178
    Wang, Quanfu
    Hou, Yanhua
    Qu, Junjie
    Hong, Yanyan
    Lin, Yifei
    Han, Xiao
    MOLECULAR BIOLOGY REPORTS, 2013, 40 (12) : 6587 - 6591
  • [32] Crystal Structure of the Apo-Form of NADPH-Dependent Thioredoxin Reductase from a Methane-Producing Archaeon
    Buey, Ruben M.
    Schmitz, Ruth A.
    Buchanan, Bob B.
    Balsera, Monica
    ANTIOXIDANTS, 2018, 7 (11)
  • [33] Expression, Purification, and Characterization of a [Fe2S2] Cluster Containing Ferredoxin from Acidithiobacillus ferrooxidans
    Jia Zeng
    Xia Huang
    Yuandong Liu
    Jianshe Liu
    Guanzhou Qiu
    Current Microbiology, 2007, 55 : 518 - 523
  • [34] The small heat shock proteins from Acidithiobacillus ferrooxidans: gene expression, phylogenetic analysis, and structural modeling
    Daniela A Ribeiro
    Luiz EV Del Bem
    Renato Vicentini
    Lúcio FC Ferraz
    Mario T Murakami
    Laura MM Ottoboni
    BMC Microbiology, 11
  • [35] Functional expression, localization, and biochemical characterization of thioredoxin glutathione reductase from air-breathing magur catfish, Clarias magur
    Koner, Debaprasad
    Nag, Niharika
    Kalita, Parismita
    Padhi, Aditya K.
    Tripathi, Timir
    Saha, Nirmalendu
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 230
  • [36] Expression, purification and spectroscopic analysis of an HdrC: An iron-sulfur cluster-containing protein from Acidithiobacillus ferrooxidans
    Ossa, D. M. H.
    Oliveira, R. R.
    Murakami, M. T.
    Vicentini, R.
    Costa-Filho, A. J.
    Alexandrino, F.
    Ottoboni, L. M. M.
    Garcia, O., Jr.
    PROCESS BIOCHEMISTRY, 2011, 46 (06) : 1335 - 1341
  • [37] Molecular cloning, expression, purification and characterization of thioredoxin from Antarctic sea-ice bacteria Pseudoalteromonas sp. AN178
    Quanfu Wang
    Yanhua Hou
    Junjie Qu
    Yanyan Hong
    Yifei Lin
    Xiao Han
    Molecular Biology Reports, 2013, 40 : 6587 - 6591
  • [38] Crude protein fraction with high thioredoxin reductase (TrxR) enzyme activity from filarial parasite Setaria cervi counters lipopolysaccharide (LPS)-induced inflammation in macrophages
    Joardar, Nikhilesh
    Jana, Kuladip
    Babu, Santi P. Sinha
    PARASITOLOGY RESEARCH, 2022, 121 (05) : 1379 - 1388
  • [39] Crude protein fraction with high thioredoxin reductase (TrxR) enzyme activity from filarial parasite Setaria cervi counters lipopolysaccharide (LPS)-induced inflammation in macrophages
    Nikhilesh Joardar
    Kuladip Jana
    Santi P. Sinha Babu
    Parasitology Research, 2022, 121 : 1379 - 1388
  • [40] Cloning, expression, purification, and kinetic characterization of mitochondrial thioredoxin (TsTrx2), cytosolic thioredoxin (TsTrx1), and glutaredoxin (TsGrx1) from Taenia solium
    Gabriela Nava
    Gerardo Maldonado
    Agustin Plancarte
    Parasitology Research, 2019, 118 : 1785 - 1797
12345