DETERMINATION OF ACYCLOVIR IN RABBIT PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC) TECHNIQUE

A rapid, sensitive and simple reversed-phase high-performance liquid chromatographic (HPLC) method has been developed and validated for the determination of acyclovir (ACV) in rabbit plasma. BDS C18 column was used to conduct analysis using ammonium dihydrogen phosphate buffer (50 mM) and methanol as mobile phase (98 : 2), with pH adjusted to 2.5 using orthophosphoric acid. The flow rate was kept at 1 mL/min. Selective precipitation of plasma proteins was done by adding 5% perchloric acid. Precipitated plasma proteins were separated by centrifugation. ACV moves in a supernatant, which was snapped and passed through a syringe filtration assembly. Direct injection of supernatant was given into a BDS C18 column and ACV was detected at 256 nm. The limit of detection for ACV in plasma was estimated as 15 ng/mL whereas the limit of quantitation was calculated as 25 ng/mL. Moreover, the developed method has been found to be selective and linear into concentration range of 25-2000 ng/mL. The present method could be successfully applied to samples from bioavailability and bioequivalence studies.