Bioleaching characteristics, influencing factors of Cu solubilization and survival of Herbaspirillum sp GW103 in Cu contaminated mine soil

被引：49

作者：

Govarthanan, Muthusamy ^{[1
]}

Lee, Gun-Woong ^{[1
]}

Park, Jung-Hee ^{[1
]}

Kim, Jae Su ^{[2
]}

Lim, Sung-Sik ^{[3
]}

Seo, Sang-Ki ^{[3
]}

Cho, Min ^{[1
]}

Myung, Hyun ^{[4
]}

Kamala-Kannan, Seralathan ^{[1
]}

Oh, Byung-Taek ^{[1
]}

机构：

[1] Chonbuk Natl Univ, Adv Inst Environm & Biosci, Div Biotechnol, Coll Environm & Bioresource Sci, Iksan 570752, South Korea

[2] Chonbuk Natl Univ, Dept Agr Biol, Coll Agr & Life Sci, Jeonju 561756, South Korea

[3] Korea Rural Community Corp, Uiwang Si 437703, Gyeonggi Do, South Korea

[4] Chonbuk Natl Univ, Dept Ecol Landscape Architecture Design, Coll Environm & Bioresource Sci, Iksan 570752, South Korea

来源：

CHEMOSPHERE | 2014年 / 109卷

基金：

新加坡国家研究基金会;

关键词：

Mine soil; Green fluorescent protein; Bioleaching; Herbaspirillum sp; Oil cake; HEAVY-METALS; BIOMINERALIZATION; IMMOBILIZATION; OPTIMIZATION; REMEDIATION; BACTERIA; LIME; LEAD; CD;

D O I：

10.1016/j.chemosphere.2014.02.054

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study was aimed at assess the potential of diazotrophic bacteria, Herbaspirillum sp. GW103, for bioleaching of Cu in mine soil. The strain exhibited resistance to As (550 mg L-1), Cu (350 mg L-1), Zn (300 mg L-1) and Pb (200 mg L-1). The copper resistance was further confirmed by locating copA and copB genes. The survival of the isolate GW103 during bioleaching was analyzed using green fluorescent protein tagged GW103. Response surface methodology based Box-Behnken design was used to optimize the physical and chemical conditions for Cu bioleaching. Five significant variables (temperature, incubation time, CaCO3 coconut oil cake (COC), agitation rate) were selected for the optimization. Second-order polynomials were established to identify the relationship between Cu bioleaching and variables. The optimal conditions for maximum Cu bioleaching (66%) were 30 degrees C, 60 h of incubation with 1.75% of CaCO3 and 3% COC at 140 rpm. The results of Cu sequential extraction studies indicated that the isolate GW103 leached Cu from ion-exchangeable, reducible, strong organic and residual fractions. Obtained results point out that the isolate GW103 could be used for bioleaching of Cu from mine soils. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：42 / 48

页数：7

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