Cryopreservation of sperm of farmed European eel Anguilla anguilla

被引:22
作者
Müller, T [1 ]
Urbányi, B
Váradi, B
Binder, T
Horn, P
Bercsényi, M
Horváth, A
机构
[1] Univ Veszprem, Georgikon Fac Agr, H-8360 Keszthely, Hungary
[2] Univ Kaposvar, Fac Anim Sci, H-7400 Kaposvar, Hungary
[3] Szent Istvan Univ, Dept Fish Culture, H-2103 Godollo, Hungary
关键词
D O I
10.1111/j.1749-7345.2004.tb01078.x
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Sexual maturation and sperm release were induced in farmed European eels Anguilla anguilla kept exclusively in fresh water by using two dosages of human chorion gonadotropin (100 International Unit (IU)-Group one and 250 IU/individual per week-Group two). Sperm release took over 13 wk in both groups. The quality of sperm was investigated on the eighth, ninth, and tenth wk. The average cell densities were 0.94 +/- 0.4 x 10(10) (Group one) and 0.93 +/- 0.6 x 10(10) (Group two) spermatozoa/mL. The estimated motility of eel sperm was 33, 55, and 49% on the eighth, ninth, and tenth wk of treatment, respectively. The estimated average motility of samples selected for cryopreservation was 73 +/- 10%, while the post-thaw motility of cryopreserved samples was 36 +/- 11%. The extender originally developed for common carp sperm crypreservation together with methanol as cryoprotectant was found suitable for the cryopreservation of European eel sperm.
引用
收藏
页码:225 / 231
页数:7
相关论文
共 31 条
[1]  
BIENIARZ K, 1977, J FISH BIOL, V10, P555
[2]   SPERMIOGENESIS AND SPERMATOZOON OF ANGUILLA-ANGUILLA L - STUDY OF ULTRASTRUCTURE [J].
BILLARD, R ;
GINSBURG, AS .
ANNALES DE BIOLOGIE ANIMALE BIOCHIMIE BIOPHYSIQUE, 1973, 13 (04) :523-+
[3]  
Billard R., 1995, Broodstock Manag. Egg Larval Qual, P25
[4]  
BOETIUS I, 1980, Dana, V1, P1
[5]  
BOETIUS INGE, 1967, MEDD DANMARKS FISKERI HAVUNDERSOGELSER, V4, P339
[6]  
DOLLERUP J, 1985, DANA-J FISH MAR RES, V4, P19
[7]   STIMULATION OF GONADOTROPIN-RELEASE AND OF OVARIAN DEVELOPMENT, BY THE ADMINISTRATION OF A GONADOLIBERIN AGONIST AND OF DOPAMINE ANTAGONISTS, IN FEMALE SILVER EEL PRETREATED WITH ESTRADIOL [J].
DUFOUR, S ;
LOPEZ, E ;
LEMENN, F ;
LEBELLE, N ;
BALOCHE, S ;
FONTAINE, YA .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1988, 70 (01) :20-30
[8]  
FONTAINE M, 1964, CR HEBD ACAD SCI, V259, P2907
[9]   CRYOPRESERVATION OF ZEBRA FISH SPERMATOZOA USING METHANOL [J].
HARVEY, B ;
KELLEY, RN ;
ASHWOODSMITH, MJ .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1982, 60 (08) :1867-1870
[10]   The effect of cryoprotectants on the motility and fertilizing capacity of cryopreserved African catfish Clarias gariepinus (Burchell 1822) sperm [J].
Horváth, A ;
Urbányi, B .
AQUACULTURE RESEARCH, 2000, 31 (03) :317-324