P-glycoprotein effect on the properties of its natural lipid environment probed by Raman spectroscopy and Langmuir-Blodgett technique

被引：9
作者：
Oleinikov, Vladimir A.
Fleury, Fabrice
Ianoul, Anatoli
Zaitsev, Sergey
Nabiev, Igor
机构：
[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Univ Nantes, CNRS, UMR 6204, Unite U3B, F-44322 Nantes 3, France
[3] Carleton Univ, Dept Chem, Ottawa, ON, Canada
[4] Univ Reims, UFR Pharm, EA 3798, IFR Biomol 53, F-51100 Reims, France
来源：
FEBS LETTERS | 2006年 / 580卷 / 20期
基金：
俄罗斯基础研究基金会;
关键词：
P-glycoprotein; multidrug resistance; Raman spectroscopy; Langmuir-Blodgett; lipid membrane;
D O I：
10.1016/j.febslet.2006.07.069
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Behavior of P-glycoprotein (Pgp) natural lipid environment within the membrane of CEM cells expressing Pgp in the quantities varying from 0% to 32% of the total amount of all membrane proteins is described for the first time. Observed cooperative effect of Pgp-induced increase of membrane stability, decrease of the temperature of gel-to-crystal lipids transition and predominance of the lipid liquid crystalline phase at physiological temperatures should have an impact in development of multidrug resistance phenotype of tumor cells by favoring the Pgp intercellular transfer and Pgp ATPase activity. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：4953 / 4958
页数：6
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