In vitro enhancement of dendritic cell-mediated anti-glioma immune response by graphene oxide

被引：22

作者：

Wang, Wei ^{[1
,2
,3
]}

Li, Zhongjun ^{[4
]}

Duan, Jinhong ^{[2
,3
]}

Wang, Chen ^{[4
]}

Fang, Ying ^{[4
]}

Yang, Xian-Da ^{[2
,3
]}

机构：

[1] Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

[2] Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China

[3] Peking Union Med Coll, Beijing 100005, Peoples R China

[4] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

来源：

NANOSCALE RESEARCH LETTERS | 2014年 / 9卷

关键词：

Glioma; Dendritic cell; DC; Graphene oxide; GO; Immunotherapy; MALIGNANT GLIOMA; SURVIVIN; PROTEIN; VACCINATION; EXPRESSION; IDENTIFICATION; GLIOBLASTOMA; INHIBITOR; DELIVERY; LYSATE;

D O I：

10.1186/1556-276X-9-311

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Malignant glioma has extremely poor prognosis despite combination treatments with surgery, radiation, and chemotherapy. Dendritic cell (DC)-based immunotherapy may potentially serve as an adjuvant treatment of glioma, but its efficacy generally needs further improvement. Here we explored whether graphene oxide (GO) nanosheets could modulate the DC-mediated anti-glioma immune response in vitro, using the T98G human glioma cell line as the study model. Pulsing DCs with a glioma peptide antigen (Ag) generated a limited anti-glioma response compared to un-pulsed DCs. Pulsing DCs with GO alone failed to produce obvious immune modulation effects. However, stimulating DCs with a mixture of GO and Ag (GO-Ag) significantly enhanced the anti-glioma immune reaction (p < 0.05). The secretion of interferon gamma (IFN-gamma) by the lymphocytes was also markedly boosted by GO-Ag. Additionally, the anti-glioma immune response induced by GO-Ag appeared to be target-specific. Furthermore, at the concentration used in this study, GO exhibited a negligible effect on the viability of the DCs. These results suggested that GO might have potential utility for boosting a DC-mediated anti-glioma immune response.

引用

页码：1 / 9

页数：9

共 37 条

[1]

Afra D, 2002, LANCET, V359, P1011

[2]

Andersen MH, 2001, CANCER RES, V61, P869

[3] Chitosan-Functionalized Graphene Oxide as a Nanocarrier for Drug and Gene Delivery [J].

Bao, Hongqian ;

Pan, Yongzheng ;

Ping, Yuan ;

Sahoo, Nanda Gopal ;

Wu, Tongfei ;

Li, Lin ;

Li, Jun ;

Gan, Leong Huat .

SMALL, 2011, 7 (11) :1569-1578

[4]

Brossart MDP, 2002, TRANSFUS APHER SCI, V27, P183

[5] Quantitatively determined survivin expression levels are of prognostic value in human gliomas [J].

Chakravarti, A ;

Noll, E ;

Black, PM ;

Finkelstein, DF ;

Finkelstein, DM ;

Dyson, NJ ;

Loeffler, JS .

JOURNAL OF CLINICAL ONCOLOGY, 2002, 20 (04) :1063-1068

[6] Antitumor effects of a xenogeneic survivin bone marrow derived dendritic cell vaccine against murine GL261 gliomas [J].

Ciesielski, Michael J. ;

Apfel, Lisa ;

Barone, Tara A. ;

Castro, Carla A. ;

Weiss, Tina C. ;

Fenstermaker, Robert A. .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 2006, 55 (12) :1491-1503

[7] The rise of graphene [J].

Geim, A. K. ;

Novoselov, K. S. .

NATURE MATERIALS, 2007, 6 (03) :183-191

[8] Treatment of malignant glioma: a problem beyond the margins of resection [J].

Giese, A ;

Westphal, M .

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2001, 127 (04) :217-225

[9] A tumor vaccine containing anti-CD3 and anti-CD28 bispecific antibodies triggers strong and durable antitumor activity in human lymphocytes [J].

Haas, C ;

Lulei, M ;

Fournier, P ;

Arnold, A ;

Schirrmacher, V .

INTERNATIONAL JOURNAL OF CANCER, 2006, 118 (03) :658-667

[10] THE FALLACY OF THE LOCALIZED SUPRATENTORIAL MALIGNANT GLIOMA [J].

HALPERIN, EC ;

BURGER, PC ;

BULLARD, DE .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1988, 15 (02) :505-509

← 1 2 3 4 →