In vitro enhancement of dendritic cell-mediated anti-glioma immune response by graphene oxide

被引：22
作者：
Wang, Wei [1 ,2 ,3 ]
Li, Zhongjun [4 ]
Duan, Jinhong [2 ,3 ]
Wang, Chen [4 ]
Fang, Ying [4 ]
Yang, Xian-Da [2 ,3 ]
机构：
[1] Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
[2] Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China
[3] Peking Union Med Coll, Beijing 100005, Peoples R China
[4] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
来源：
NANOSCALE RESEARCH LETTERS | 2014年 / 9卷
关键词：
Glioma; Dendritic cell; DC; Graphene oxide; GO; Immunotherapy; MALIGNANT GLIOMA; SURVIVIN; PROTEIN; VACCINATION; EXPRESSION; IDENTIFICATION; GLIOBLASTOMA; INHIBITOR; DELIVERY; LYSATE;
D O I：
10.1186/1556-276X-9-311
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Malignant glioma has extremely poor prognosis despite combination treatments with surgery, radiation, and chemotherapy. Dendritic cell (DC)-based immunotherapy may potentially serve as an adjuvant treatment of glioma, but its efficacy generally needs further improvement. Here we explored whether graphene oxide (GO) nanosheets could modulate the DC-mediated anti-glioma immune response in vitro, using the T98G human glioma cell line as the study model. Pulsing DCs with a glioma peptide antigen (Ag) generated a limited anti-glioma response compared to un-pulsed DCs. Pulsing DCs with GO alone failed to produce obvious immune modulation effects. However, stimulating DCs with a mixture of GO and Ag (GO-Ag) significantly enhanced the anti-glioma immune reaction (p < 0.05). The secretion of interferon gamma (IFN-gamma) by the lymphocytes was also markedly boosted by GO-Ag. Additionally, the anti-glioma immune response induced by GO-Ag appeared to be target-specific. Furthermore, at the concentration used in this study, GO exhibited a negligible effect on the viability of the DCs. These results suggested that GO might have potential utility for boosting a DC-mediated anti-glioma immune response.
引用
收藏
页码：1 / 9
页数：9
相关论文
共 37 条
  • [1] Afra D, 2002, LANCET, V359, P1011
  • [2] Andersen MH, 2001, CANCER RES, V61, P869
  • [3] Chitosan-Functionalized Graphene Oxide as a Nanocarrier for Drug and Gene Delivery
    Bao, Hongqian
    Pan, Yongzheng
    Ping, Yuan
    Sahoo, Nanda Gopal
    Wu, Tongfei
    Li, Lin
    Li, Jun
    Gan, Leong Huat
    [J]. SMALL, 2011, 7 (11) : 1569 - 1578
  • [4] Brossart MDP, 2002, TRANSFUS APHER SCI, V27, P183
  • [5] Quantitatively determined survivin expression levels are of prognostic value in human gliomas
    Chakravarti, A
    Noll, E
    Black, PM
    Finkelstein, DF
    Finkelstein, DM
    Dyson, NJ
    Loeffler, JS
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 2002, 20 (04) : 1063 - 1068
  • [6] Antitumor effects of a xenogeneic survivin bone marrow derived dendritic cell vaccine against murine GL261 gliomas
    Ciesielski, Michael J.
    Apfel, Lisa
    Barone, Tara A.
    Castro, Carla A.
    Weiss, Tina C.
    Fenstermaker, Robert A.
    [J]. CANCER IMMUNOLOGY IMMUNOTHERAPY, 2006, 55 (12) : 1491 - 1503
  • [7] The rise of graphene
    Geim, A. K.
    Novoselov, K. S.
    [J]. NATURE MATERIALS, 2007, 6 (03) : 183 - 191
  • [8] Treatment of malignant glioma: a problem beyond the margins of resection
    Giese, A
    Westphal, M
    [J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2001, 127 (04) : 217 - 225
  • [9] A tumor vaccine containing anti-CD3 and anti-CD28 bispecific antibodies triggers strong and durable antitumor activity in human lymphocytes
    Haas, C
    Lulei, M
    Fournier, P
    Arnold, A
    Schirrmacher, V
    [J]. INTERNATIONAL JOURNAL OF CANCER, 2006, 118 (03) : 658 - 667
  • [10] THE FALLACY OF THE LOCALIZED SUPRATENTORIAL MALIGNANT GLIOMA
    HALPERIN, EC
    BURGER, PC
    BULLARD, DE
    [J]. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1988, 15 (02): : 505 - 509
1234