Selective voltammetric and flow-injection determination of guanine and adenine on a glassy carbon electrode modified by a ruthenium hexachloroplatinate film

被引:7
作者
Shaidarova, L. G. [1 ]
Gedmina, A. V. [1 ]
Zhaldak, E. R. [1 ]
Chelnokova, I. A. [1 ]
Budnikov, G. K. [1 ]
机构
[1] Kazan Fed Univ, Butlerov Inst Chem, Kazan 420008, Russia
基金
俄罗斯基础研究基金会;
关键词
chemically modified electrodes; inorganic polymer films; ruthenium hexachloroplatinate; guanine; adenine; electrocatalysis; electroanalysis; flow-injection analysis; ELECTROCATALYTIC PROPERTIES; COMPOSITE FILM; ELECTROCHEMICAL PREPARATION; OXIDATION; DNA; NAD(+); ACIDS;
D O I
10.1134/S1061934814080097
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An inorganic film of ruthenium hexachloroplatinate (RuPtCl6), deposited on the surface of a glassy carbon electrode, exhibits electrocatalytic activity in the oxidation of purine bases, such as adenine and guanine. Appropriate working conditions are found for fabricating a polymer film on the surface of glassy carbon and for recording the maximum electrocatalytic effect for the modified electrode. A method is developed for the selective voltammetric determination of guanine and adenine in their simultaneous presence on an electrode modified by a RuPtCl6 film. A procedure is proposed for the amperometric detection of purine bases with this modified electrode under the conditions of flow-injection analysis. The dependence of the analytical signal on the concentration of guanine and adenine is linear up to 5 x 10(-6) and 5 x 10(-7) M in the stationary mode and to 5 x 10(-7) and 5 x 10(-8) M under flow conditions, respectively. The proposed method was tested in the analysis of calf thymus DNA for the concentrations of guanine and adenine.
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页码:741 / 749
页数:9
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