Poly(N-isopropylacrylamide)-gated Fe3O4/SiO2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme

被引：48

作者：

Yu, Erick ^{[1
,3
]}

Galiana, Irene ^{[1
]}

Martinez-Manez, Ramon ^{[1
,2
]}

Stroeve, Pieter ^{[3
]}

Marcos, Maria D. ^{[1
,2
]}

Aznar, Elena ^{[2
]}

Sancenon, Felix ^{[1
,2
]}

Murguia, Jose R. ^{[1
]}

Amoros, Pedro ^{[4
]}

机构：

[1] Univ Politecn Valencia, Univ Valencia, Dept Quim,Unidad Mixta, Ctr Reconocimiento Mol & Desarrollo Tecnol IDM, E-46022 Valencia, Spain

[2] CIBER Bioingn Biomat & Nanomed CIBER BBN, Valencia, Spain

[3] Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA

[4] Univ Valencia, Inst Ciencia Mat ICMUV, Valencia 46071, Spain

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 135卷

关键词：

Protein delivery; Triggered release; Mesoporous silica; Pore expansion; PNIPAM; RESPONSIVE CONTROLLED-RELEASE; SILICA NANOPARTICLES; DRUG-DELIVERY; GUEST MOLECULES; NANOCONTAINERS; SYSTEM; CARGO; GENE;

D O I：

10.1016/j.colsurfb.2015.06.048

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Core-shell nanoparticles comprised of Fe3O4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(N-isopropylacrylamide) (PNIPAM) layer (CS-MSNs-EP-PNIPAM) were prepared and characterized. The nanoparticles was loaded with (Ru(bipy)(3)(2+)) dye or an antibacterial enzyme, lysozyme, to obtain CS-MSNs-EP-PNIPAM-Ru(bipy)(3)(2+) and CS-MSNs-EP-PNIPAM-Lys, respectively. The lysozyme loading was determined to be 160 mg/g of nanoparticle. It was seen that Ru(bipy)(3)(2+) and lysozyme release was minimal at a room temperature of 25 degrees C while at physiological temperature (37 degrees C), abrupt release was observed. The applicability of the CS-MSNs-EP-PNIPAM-Lys was further tested with two Gram-positive bacteria samples, Bacillus cereus and Micrococcus luteus. At physiological temperature, the nanoparticles were shown to reduce bacterial growth, indicating a successful release of lysozyme from the nanoparticles. This nanoparticle system shows potential as a nanocarrier for the loading of similarly sized proteins or other species as a drug delivery platform. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：652 / 660

页数：9