Evaluation of simple enzyme kinetics by response surface modelling

被引：28

作者：

Andersson, M ^{[1
]}

Adlercreutz, P ^{[1
]}

机构：

[1] Univ Lund, Dept Biotechnol, Ctr Chem & Chem Engn, S-22100 Lund, Sweden

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 12期

关键词：

alcohol dehydrogenase; cyclohexanone; enzyme kinetics; response surface modelling;

D O I：

10.1023/A:1008994613645

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity of a horse liver alcohol dehydrogenase catalysed reduction of cyclohexanone was investigated by using a central composite circumscribed design in which two parameters (pH and cyclohexanone concentration) were varied. By log transformation of the substrate concentration an adequate model could be obtained from which reliable kinetic constants and pH profiles were determined.

引用

页码：903 / 907

页数：5