Calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields in U87 human glioblastoma cells

被引：44

作者：

Carr, Lynn ^{[1
]}

Bardet, Sylvia M. ^{[1
]}

Burke, Ryan C. ^{[1
]}

Arnaud-Cormos, Delia ^{[1
]}

Leveque, Philippe ^{[1
]}

O'Connor, Rodney P. ^{[1
,2
]}

机构：

[1] Univ Limoges, Fac Sci & Tech, XLIM Res Inst, CNRS,UMR 7252, 123 Ave Albert Thomas, F-87060 Limoges, France

[2] Ecole Natl Super Mines, Ctr Microelect Provence, Bioelect Dept, Georges Charpak Campus,880 Route Mimet, F-13541 Gardanne, France

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

PLASMA-MEMBRANE; ACTIVATION; TUBULIN; INHIBITION; PERMEABILIZATION; MITOCHONDRIA; CYTOSKELETON; ASSOCIATION; ULTRASOUND; MICROSCOPY;

D O I：

10.1038/srep41267

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

High powered, nanosecond duration, pulsed electric fields (nsPEF) cause cell death by a mechanism that is not fully understood and have been proposed as a targeted cancer therapy. Numerous chemotherapeutics work by disrupting microtubules. As microtubules are affected by electrical fields, this study looks at the possibility of disrupting them electrically with nsPEF. Human glioblastoma cells (U87-MG) treated with 100, 10 ns, 44 kV/cm pulses at a frequency of 10 Hz showed a breakdown of their interphase microtubule network that was accompanied by a reduction in the number of growing microtubules. This effect is temporally linked to loss of mitochondrial membrane potential and independent of cellular swelling and calcium influx, two factors that disrupt microtubule growth dynamics. Super-resolution microscopy revealed microtubule buckling and breaking as a result of nsPEF application, suggesting that nsPEF may act directly on microtubules.

引用

页数：12

共 73 条

[31] Voltage-dependent Anion Channels Modulate Mitochondrial Metabolism in Cancer Cells REGULATION BY FREE TUBULIN AND ERASTIN [J].