dPCR vs. qPCR: The role of Poisson statistics at low concentrations

被引：9
作者：
Tellinghuisen, Joel [1 ]
机构：
[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2020年 / 611卷
关键词：
dPCR; qPCR; Data analysis; Poisson statistics; Statistical precision;
D O I：
10.1016/j.ab.2020.113946
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The ultimate precision in both dPCR and qPCR experiments is limited by the Poisson statistics in the total number m of template molecules in the sample, giving relative standard deviation 1/root m . This means that precision is limited by sample volume at low concentrations. Accordingly qPCR instruments, used in dPCR mode, can give better precision than dPCR instruments in this limit. For example, 13% standard deviation can be achieved with a 96-well plate for number concentrations similar to 20-5000 mL(-1). For fixed m, qPCR loses to dPCR by a factor of-2 in precision when calibration is needed.
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