Utilisation of chickpea protein isolates for production of peptides with angiotensin I-converting enzyme (ACE)-inhibitory activity

被引：161

作者：

Pedroche, J ^{[1
]}

Yust, MM ^{[1
]}

Girón-Calle, J ^{[1
]}

Alaiz, M ^{[1
]}

Millán, F ^{[1
]}

Vioque, J ^{[1
]}

机构：

[1] Inst Grasa, E-41012 Seville, Spain

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2002年 / 82卷 / 09期

关键词：

chickpea protein hydrolysate; ACE inhibitors; biological peptides;

D O I：

10.1002/jsfa.1126

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Chickpea protein isolates and the protease alcalase were used for the production of protein hydrolysates that inhibit angiotensin I-converting enzyme (ACE). The highest degree of inhibition was found in a hydrolysate obtained by 30 min of treatment with alcalase. This hydrolysate was used as starting material for the purification of ACE-inhibitory peptides. After Biogel P2 gel filtration chromatography and HPLC C-18 reverse phase chromatography, four peptides with ACE-inhibitory activity were purified. Two of them were competitive inhibitors of ACE, while the other two were uncompetitive inhibitors. These results show that chickpea proteins are a good source of ACE-inhibitory peptides when hydrolysed with the protease alcalase. (C) 2002 Society of Chemical Industry.

引用

页码：960 / 965

页数：6

共 45 条

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